{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:44:14Z","timestamp":1791182654564,"version":"4.1.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":1927,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T00:00:00Z","timestamp":1600214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016512","name":"Center for Companion Animal Health, University of California, Davis","doi-asserted-by":"publisher","award":["2019-30-F"],"award-info":[{"award-number":["2019-30-F"]}],"id":[{"id":"10.13039\/100016512","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Maxine Adler Graduate Fellowship for Eric Ontiveros"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>A nonpedigreed male cat presented with epistaxis, severe bladder hemorrhage, and secondary urethral obstruction after cystocentesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To characterize the phenotype of a cat with bleeding diathesis and use a precision medicine approach to identify the molecular genetic defect by whole genome sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Adenosine diphosphate (ADP) and arachidonic acid (AA)-induced whole blood platelet aggregometry was performed in the affected cat and a healthy cat. Platelet activation, measured by P-selectin expression, and surface integrin subunit \u03b23 expression were evaluated by flow cytometry in the affected cat and healthy control. Total integrin subunit \u03b1IIb expression was assessed by western blot. Whole genome sequencing at 30\u00d7 coverage was used to identify genetic variants that segregated in the affected cat compared to 194 cats from the 99 Lives Sequencing Consortium.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Platelet aggregometry identified significant impairment in platelet aggregation in response to ADP and AA compared to the control cat. Targeted protein expression analyses by flow cytometry and immunoblot analysis determined that the surface expression and total expression of the integrin, \u03b1IIb\u03b23, was absent. Whole genome sequencing identified a homozygous c.1986delC frameshift variant in the integrin subunit \u03b1IIb (ITGA2B) gene that was not detected in the control population. The p.Pro662fs (ITGA2B P662X) variant terminates translation of the protein at the extracellular domain of the integrin prematurely, which is predicted to affect expression of the \u03b23 unit.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>This novel ITGA2B variant and the associated phenotype closely resemble Glanzmann's thrombasthenia, which has never been reported in cats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.15886","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T16:38:26Z","timestamp":1600274306000},"page":"2438-2446","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Precision medicine identifies a pathogenic variant of the\n                    <i>ITGA2B<\/i>\n                    gene responsible for Glanzmann's thrombasthenia in a cat"],"prefix":"10.1093","volume":"34","author":[{"name":"the 99 Lives Cat Genome Consortium","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3325-2744","authenticated-orcid":false,"given":"Ronald H. L.","family":"Li","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis Department of Veterinary Surgical and Radiological Sciences, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Ontiveros","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis Department of Veterinary Medicine and Epidemiology, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nghi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis Department of Veterinary Surgical and Radiological Sciences, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua A.","family":"Stern","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis Department of Veterinary Medicine and Epidemiology, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Lee","sequence":"additional","affiliation":[{"name":"Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian T.","family":"Hardy","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis Department of Veterinary Medicine and Epidemiology, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,11,1]]},"reference":[{"key":"2026020922093063200_jvim15886-bib-0001","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1354\/vp.38-3-249","article-title":"Clinical, biochemical, and molecular aspects of 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