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Damaged axons of the central nervous system release NfLs into the cerebrospinal fluid (CSF) and the blood. In humans with neurologic diseases, NfL is used as a biomarker.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To identify the potential of NfL as a supportive tool for the diagnosis, prognosis, and monitoring of meningoencephalitis of unknown etiology (MUE) in dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twenty-six client-owned healthy dogs, 10 normal Beagle dogs, and 38 client-owned MUE dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cohort study. The concentrations of NfL in serum and CSF were measured using single-molecule array technology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Median NfL concentration was significantly higher in MUE dogs (serum, 125\u2009pg\/mL; CSF, 14\u2009700\u2009pg\/mL) than in healthy dogs (serum, 11.8\u00a0pg\/mL, P\u00a0&amp;lt;\u2009.0001; CSF, 1410\u2009pg\/mL, P\u00a0=\u2009.0002). The areas under the receiver operating characteristic curves of serum and CSF NfL concentrations were 0.99 and 0.95, respectively. The cut-off values were 41.5\u00a0pg\/mL (serum) and 4005\u2009pg\/mL (CSF) for differentiating between healthy and MUE dogs, with sensitivities of 89.19% and 90%, respectively, and specificities of 96.97% and 100%, respectively. The NfL concentration showed a significant decrease (pretreatment, 122\u2009pg\/mL; posttreatment, 36.6\u00a0pg\/mL; P\u00a0=\u2009.02) in the good treatment-response group and a significant increase (pretreatment, 292.5\u00a0pg\/mL; posttreatment, 1880\u2009pg\/mL, P\u00a0=\u2009.03) in the poor treatment-response group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical importance<\/jats:title>\n                    <jats:p>Neurofilament light chain is a potential biomarker for diagnosing MUE and evaluating response to treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.16184","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T02:30:36Z","timestamp":1623378636000},"page":"1865-1872","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Neurofilament light chain as a biomarker of meningoencephalitis of unknown etiology in dogs"],"prefix":"10.1093","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1372-4430","authenticated-orcid":false,"given":"Taesik","family":"Yun","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University , Cheongju, Chungbuk,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3810-4193","authenticated-orcid":false,"given":"Yoonhoi","family":"Koo","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University , Cheongju, Chungbuk,","place":["Republic of 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Medicine, College of Veterinary Medicine, Chungbuk National University , Cheongju, Chungbuk,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6739-9787","authenticated-orcid":false,"given":"Soochong","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chungbuk National University , Cheongju, Chungbuk,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7721-773X","authenticated-orcid":false,"given":"Dongwoo","family":"Chang","sequence":"additional","affiliation":[{"name":"Section of Veterinary Medical Imaging, Veterinary Teaching Hospital, College of Veterinary Medicine, Chungbuk National University , Cheongju, Chungbuk,","place":["Republic of 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