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Complete cardiac assessment, flow cytometry (T lymphocytes [CD3+], their subtypes [CD3+CD4+, CD3+CD8+, CD3+CD4+CD8+, CD3+CD4\u2212CD8\u2212], and B lymphocytes [CD45+CD21+]) and measurement of N-terminal pro B-type natriuretic peptide, cardiac troponin I, and C-reactive protein concentrations were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The percentage of CD3+CD4+ lymphocytes was significantly lower in stable ACVIM C patients (P\u00a0=\u00a0.01) and unstable ACVIM C and D patients (P\u00a0=\u00a0.003), the percentage of CD3+CD8+ lymphocytes was significantly higher in stable ACVIM C patients (P\u00a0=\u00a0.01) and unstable ACVIM C and D patients (P\u00a0=\u00a0.01), CD3+CD8+ lymphocyte concentration was significantly higher in unstable ACVIM C and D patients (P\u00a0=\u00a0.05), and the CD3+CD4+\/CD3+CD8+ ratio was significantly lower in stable ACVIM C patients (P\u00a0=\u00a0.01) and unstable ACVIM C and D patients (P\u00a0=\u00a0.01) compared with healthy controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>The percentages of CD3+CD4+ and CD3+CD8+ PBL and CD4+\/CD8+ ratio were altered in MMVD dogs with congestive heart failure (ACVIM C, D), but not in ACVIM B2, suggesting involvement of these PBL subtypes in the pathogenesis of congestive heart failure in dogs with MMVD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.16207","type":"journal-article","created":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T06:46:54Z","timestamp":1625726814000},"page":"2112-2122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Peripheral blood lymphocyte subtypes in dogs with different stages of myxomatous mitral valve disease"],"prefix":"10.1093","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7717-1524","authenticated-orcid":false,"given":"Natalia","family":"Druzhaeva","sequence":"first","affiliation":[{"name":"Small Animal Clinic, Veterinary Faculty, University of Ljubljana , Ljubljana,","place":["Slovenia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1199-4501","authenticated-orcid":false,"given":"Alenka","family":"Nemec Svete","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Veterinary Faculty, University of Ljubljana , Ljubljana,","place":["Slovenia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alojz","family":"Ihan","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana , Ljubljana,","place":["Slovenia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katka","family":"Pohar","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana , Ljubljana,","place":["Slovenia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5636-9163","authenticated-orcid":false,"given":"Aleksandra","family":"Domanjko Petri\u010d","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Veterinary Faculty, University of Ljubljana , Ljubljana,","place":["Slovenia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,9,1]]},"reference":[{"issue":"5","key":"2026020303471025900_jvim16207-bib-0001","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1111\/jvim.15488","article-title":"ACVIM consensus guidelines for the diagnosis and treatment of myxomatous mitral valve disease in dogs","volume":"33","author":"Keene","year":"2019","journal-title":"J Vet Intern Med"},{"issue":"5","key":"2026020303471025900_jvim16207-bib-0002","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1007\/BF02938356","article-title":"Role of inflammation in the progression of heart failure","volume":"9","author":"Yndestad","year":"2007","journal-title":"Curr Cardiol Rep"},{"issue":"5","key":"2026020303471025900_jvim16207-bib-0003","doi-asserted-by":"crossref","first-page":"682","DOI":"10.2174\/1573406410666140318113325","article-title":"Inflammation and chronic heart failure: from biomarkers to novel anti-inflammatory therapeutic strategies","volume":"10","author":"Bouras","year":"2014","journal-title":"Med Chem"},{"issue":"5","key":"2026020303471025900_jvim16207-bib-0004","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s11897-017-0337-9","article-title":"Inflammation - 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