{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:27:27Z","timestamp":1787502447841,"version":"build-2736575974"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":1435,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T00:00:00Z","timestamp":1642636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Boehringer Ingelheim Vetmedica, Inc","award":["PR0481"],"award-info":[{"award-number":["PR0481"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diagnosis of pituitary pars intermedia dysfunction (PPID) using the thyrotropin-releasing hormone (TRH) stimulation test requires blood collection 10\u00a0minutes after TRH injection; it is unknown if small differences in timing affect test results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine whether early or late sampling results in a significant (\u226510%) difference in plasma adrenocorticotropic hormone (ACTH) concentration compared to standard 10-minute sampling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twenty-four healthy adult horses with unknown PPID status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this prospective study, subjects underwent a single TRH stimulation test, with blood collected exactly 9\u00a0minutes (early), 10\u00a0minutes (standard), and 11\u2009minutes (late) after injection. ACTH was measured by chemiluminescent immunoassay. Two aliquots of the 10-minute plasma sample were analyzed separately to assess intra-assay variability. Data were reported descriptively and bias was calculated using Bland-Altman plots. Significance was set at P\u00a0=\u00a0.05.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Minor variability was observed between the paired 10-minute sample aliquots (range, 0%-6%; median 3%). Overall variability of early or late samples compared to the corresponding paired (average) 10-minute standard concentration ranged from 0% to 92% (median 10%). Seventy-five percent of horses (18\/24) tested had at least 1 early or late reading that differed by \u226510% from its corresponding 10-minute standard concentration, and 21% of horses (5\/24) would have a different interpretation of testing result with either early or late sampling. Incidence of \u226510% variability was independent of PPID status (P\u00a0=\u00a0.59).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Precise timing of sample collection is critical to ensure accurate assessment of PPID status given the observation of significant variability associated with minor alterations in timing of sample collection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.16362","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T05:47:49Z","timestamp":1642657669000},"page":"770-777","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of early or late blood sampling on thyrotropin releasing hormone stimulation test results in horses"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-7340","authenticated-orcid":false,"given":"Kristen","family":"Thane","sequence":"first","affiliation":[{"name":"Tufts Cummings School of Veterinary Medicine , Department of Comparative Pathobiology, North Grafton, Massachusetts,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Uricchio","sequence":"additional","affiliation":[{"name":"University of Massachusetts , Department of Veterinary and Animal Sciences, Amherst, Massachusetts,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0564-7976","authenticated-orcid":false,"given":"Nicholas","family":"Frank","sequence":"additional","affiliation":[{"name":"Tufts Cummings School of Veterinary Medicine , Department of Comparative Pathobiology, North Grafton, 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