{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T05:59:57Z","timestamp":1793253597536,"version":"4.5.0"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":1313,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T00:00:00Z","timestamp":1654819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015226","name":"Royal Veterinary College","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015226","name":"Royal Veterinary College","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In dogs, 6 single-nucleotide polymorphisms (SNPs) have been described in the glucocorticoid receptor gene NR3C1a, 2 of which were nonsynonymous SNPs in exons 2 and 8. The clinical importance of these SNPs is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate whether SNPs in NR3C1a are associated with clinical outcome in Cocker Spaniels with primary immune thrombocytopenia (pITP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twenty-four Cocker Spaniels with pITP presented to a referral center. Dogs were classified as slow (n\u00a0=\u00a011) or fast responders (n\u00a0=\u00a012) based on time required after initiating glucocorticoid treatment to achieve a platelet count &amp;gt;70\u2009000\/\u03bcL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Deoxyribonucleic acid was extracted from stored blood samples before amplification by PCR and sequencing of exons 2 and 8 of NR3C1a. Associations between genotype and clinical response variables were investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Neither previously identified nonsynonymous SNPs were identified. The synonymous SNP NR3C1a:c.798C&amp;gt;T in exon 2 was found at an increased prevalence compared to a previous report. No difference was found in prevalence of any genotype at NR3C1a:c.798C&amp;gt;T between fast and slow responders (P\u00a0=\u00a0.70).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>None of the previously reported nonsynonymous SNPs in exons 2 and 8 of the NR3C1a gene were detected in our cohort of Cocker Spaniels with pITP. The synonymous SNP NR3C1a:c.798C&amp;gt;T in exon 2 was reported at a higher frequency than previously, but was not associated with outcome measures that estimated responsiveness to glucocorticoids.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.16468","type":"journal-article","created":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T01:18:08Z","timestamp":1654910288000},"page":"1281-1286","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation of single-nucleotide polymorphisms in the\n                    <i>NR3C1a<\/i>\n                    glucocorticoid receptor gene in Cocker Spaniels with primary immune thrombocytopenia"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7563-8672","authenticated-orcid":false,"given":"Sarah","family":"Tayler","sequence":"first","affiliation":[{"name":"Royal Veterinary College Department of Clinical Science and Services, , Hatfield,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7185-0446","authenticated-orcid":false,"given":"Katarina","family":"Hazuchova","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Internal Medicine, Justus-Liebig-University of Giessen , Giessen,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Riddle","sequence":"additional","affiliation":[{"name":"Clinical Investigation Centre, Royal Veterinary College , Hatfield,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7988-9997","authenticated-orcid":false,"given":"James W.","family":"Swann","sequence":"additional","affiliation":[{"name":"Royal Veterinary College Department of Clinical Science and Services, , Hatfield,","place":["United Kingdom"]},{"name":"Columbia Stem Cell Initiative, Columbia University 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