{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:15:58Z","timestamp":1787015758544,"version":"build-2736575974"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":1313,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Alvin and June Perlman Endowment in Animal Oncology"},{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["129819-IRG-16-189-58-IRG-114"],"award-info":[{"award-number":["129819-IRG-16-189-58-IRG-114"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["857424"],"award-info":[{"award-number":["857424"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Animal Cancer Care and Research Program of the University of Minnesota"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K01-OD019912"],"award-info":[{"award-number":["K01-OD019912"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K01-OD027058"],"award-info":[{"award-number":["K01-OD027058"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["129819\u2010IRG\u201016\u2010189\u201058\u2010IRG\u2010114"],"award-info":[{"award-number":["129819\u2010IRG\u201016\u2010189\u201058\u2010IRG\u2010114"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["857424"],"award-info":[{"award-number":["857424"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K01\u2010OD019912"],"award-info":[{"award-number":["K01\u2010OD019912"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K01\u2010OD027058"],"award-info":[{"award-number":["K01\u2010OD027058"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Myelofibrosis often lacks an identifiable cause in dogs. In humans, most primary myelofibrosis cases develop secondary to driver mutations in JAK2, CALR, or MPL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine the prevalence of variants in JAK2, CALR, or MPL candidate regions in dogs with myelofibrosis and in healthy dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twenty-six dogs with myelofibrosis that underwent bone marrow biopsy between 2010 and 2018 and 25 control dogs matched for age, sex, and breed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cross-sectional study. Amplicon sequencing of JAK2 exons 12 and 14, CALR exon 9, and MPL exon 10 was performed on formalin-fixed, decalcified, paraffin-embedded bone marrow (myelofibrosis) or peripheral blood (control) DNA. Somatic variants were categorized as likely-benign or possibly-pathogenic based on predicted impact on protein function. Within the myelofibrosis group, hematologic variables and survival were compared by variant status (none, likely-benign only, and\u2009\u22651 possibly-pathogenic). The effect of age on variant count was analyzed using linear regression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Eighteen of 26 (69%) myelofibrosis cases had somatic variants, including 9 classified as possibly-pathogenic. No somatic variants were detected in controls. Within the myelofibrosis group, hematologic variables and survival did not differ by variant status. The number of somatic variants per myelofibrosis case increased with age (estimate, 0.69; SE, 0.29; P\u00a0=\u2009.03).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Somatic variants might initiate or perpetuate myelofibrosis in dogs. Our findings suggest the occurrence of clonal hematopoiesis in dogs, with increasing incidence with age, as observed in humans.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.16476","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T07:58:32Z","timestamp":1657526312000},"page":"1237-1247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Targeted sequencing of candidate gene regions for myelofibrosis in dogs"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9769-4728","authenticated-orcid":false,"given":"Amelia G.","family":"Campbell","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, University of Minnesota Department of Veterinary Clinical Sciences, , St. Paul, Minnesota,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1733-8177","authenticated-orcid":false,"given":"Davis M.","family":"Seelig","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, University of Minnesota Department of Veterinary Clinical Sciences, , St. Paul, Minnesota,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan D.","family":"Beckman","sequence":"additional","affiliation":[{"name":"Division of Hematology, Oncology and Transplantation, Medical School, University of Minnesota , Minneapolis, Minnesota,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie M.","family":"Minor","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, University of Minnesota Department of Veterinary Clinical Sciences, , St. Paul, Minnesota,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Heinrich","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, University of Minnesota Department of Veterinary Clinical Sciences, , St. Paul, Minnesota,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7510-2322","authenticated-orcid":false,"given":"Steven G.","family":"Friedenberg","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, University of Minnesota Department of Veterinary Clinical Sciences, , St. Paul, Minnesota,","place":["USA"]},{"name":"Animal Cancer Care and Research Program, University of Minnesota , St. Paul, Minnesota,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime F.","family":"Modiano","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, University of Minnesota Department of Veterinary Clinical Sciences, , St. Paul, Minnesota,","place":["USA"]},{"name":"Animal Cancer Care and Research Program, University of Minnesota , St. Paul, Minnesota,","place":["USA"]},{"name":"Masonic Cancer Center, University of Minnesota , Minneapolis, Minnesota,","place":["USA"]},{"name":"Medical School, University of Minnesota Department of Laboratory Medicine and Pathology, , Minneapolis, Minnesota,","place":["USA"]},{"name":"Center for Immunology, University of Minnesota , Minneapolis, Minnesota,","place":["USA"]},{"name":"Stem Cell Institute, University of Minnesota , Minneapolis, Minnesota,","place":["USA"]},{"name":"Institute for Engineering in Medicine, University of Minnesota , Minneapolis, Minnesota,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7339-2354","authenticated-orcid":false,"given":"Eva","family":"Furrow","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, University 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