{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:27:53Z","timestamp":1790623673714,"version":"4.1.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":1009,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"AL4AnimalS","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00276\/2020"],"award-info":[{"award-number":["UIDB\/00276\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00276\/2020"],"award-info":[{"award-number":["UIDB\/00276\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pheochromocytoma (PCC) is rare in cats and plasma (PL) and urinary (U) metanephrines (metanephrine [MN]; normetanephrine [NMN]) measurement is rarely described in cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We evaluated the utility of PL and U MNs measurement in 10 healthy cats and a cat with a confirmed diagnosis of pheochromocytoma (PheoCat), using liquid chromatography with tandem mass spectrometry (LC-MS-MS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Urine and EDTA PL samples collected from each of the 10 cats and the PheoCat were promptly stored at \u221280\u00b0C and remained frozen until analysis. To evaluate U MNs stability, an additional urine sample collected from the healthy cats was refrigerated for 24\u2009hours before freezing. Urinary creatinine concentration (Creat) was assessed using the same spot urine samples to calculate U MNs-to-creatinine ratios.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The PL-MN and PL-NMN median concentrations of the healthy cats were 2.73 and 7.02\u2009nmol\/L, respectively. The median U-MN\/Creat and U-NMN\/Creat ratios were 70 and 139\u2009\u03bcg\/g, respectively. The PheoCat had a PL-MN of 3.68\u2009nmol\/L, PL-NMN of 66.27\u2009nmol\/L, U-MN\/Creat of 179\u2009\u03bcg\/g, and U-NMN\/Creat of 1262\u2009\u03bcg\/g. The PheoCat had markedly increased concentrations of both PL and U MNs when compared to the healthy cats. No significant difference was found between U MNs measured in urine samples that underwent 24\u2009hours of refrigeration in comparison to those that were frozen immediately.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We report preliminary reference intervals for PL and U MNs in cats using LC-MS-MS and the potential clinical applicability of these biomarkers for the diagnosis of PCC in cats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.16648","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T02:42:43Z","timestamp":1681440163000},"page":"910-914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Plasma and urinary metanephrine and normetanephrine concentrations using liquid chromatography with tandem mass spectrometry in healthy cats and in a cat with pheochromocytoma"],"prefix":"10.1093","volume":"37","author":[{"given":"Maria Teresa","family":"Prego","sequence":"first","affiliation":[{"name":"Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Lisbon , Lisbon,","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Joana","family":"Dias","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Lisbon , Lisbon,","place":["Portugal"]},{"name":"CIISA \u2013 Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculty of Veterinary Medicine, University of Lisbon , Lisbon,","place":["Portugal"]},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tr\u00e1s-os-Montes e Alto Douro , Vila Real,","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui Lemos","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Lisbon , Lisbon,","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salom\u00e9","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"CIISA \u2013 Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculty of Veterinary Medicine, University of Lisbon , Lisbon,","place":["Portugal"]},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tr\u00e1s-os-Montes e Alto Douro , Vila Real,","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago","family":"Dias Domingues","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, University of Lisbon Department of Statistics and Operations Research, , Lisbon,","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greet","family":"Junius","sequence":"additional","affiliation":[{"name":"AML \u2013 Algemeen Medisch Laboratorium , Antwerpen,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evi","family":"Van den Steen","sequence":"additional","affiliation":[{"name":"AML \u2013 Algemeen Medisch Laboratorium , Antwerpen,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9159-5596","authenticated-orcid":false,"given":"Sara","family":"Galac","sequence":"additional","affiliation":[{"name":"Utrecht University Department of Clinical Sciences, , Utrecht,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2463-4062","authenticated-orcid":false,"given":"Rodolfo Oliveira","family":"Leal","sequence":"additional","affiliation":[{"name":"Veterinary 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