{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T22:09:32Z","timestamp":1770156572722,"version":"3.49.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":704,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Firestone\/Tamworth\/Raker-Tulleners Grant","award":["1-400666-xxxx-2000-5919"],"award-info":[{"award-number":["1-400666-xxxx-2000-5919"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Eight-hydroxy-2\u2032-deoxyguanosine (8-OHdG), a biomarker of oxidative damage evaluated in human neurodegenerative disease, has potential to correlate with postmortem diagnosis of neuroaxonal dystrophy\/degenerative myeloencephalopathy (NAD\/DM) in horses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis<\/jats:title>\n                    <jats:p>We hypothesized that 8-OHdG will be higher in CSF and serum from NAD\/DM horses compared with horses with other neurologic diseases (CVSM, EPM) and a control group of neurologically normal horses. We also hypothesized that 8-OHdG will be higher in CSF compared with serum from NAD\/DM horses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Fifty client-owned horses with postmortem diagnoses: 20 NAD\/DM, 10 CVSM, 10 EPM, and 10 control horses. Serum and CSF samples were obtained between November 2010 and March 2022.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Case-control study using biobanked samples was performed and commercial competitive ELISA kit (Highly Sensitive 8-OHdG Check ELISA) utilized. Concentration of 8-OHdG was quantitated in both CSF and serum and compared between groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>No correlation was established between the measures of 8-OHdG in serum and CSF and group. CSF median [8-OHdG] for NAD\/DM was 169.9\u2009pg\/mL (IQR25-75: 67.18-210.6), CVSM 157.1\u2009pg\/mL (IQR25-75: 132.1-229.1), EPM 131.4\u2009pg\/mL (IQR25-75: 102.1-193.2), and control 149.8\u2009pg\/mL (IQR25-75: 113.3-196.4). Serum median [8-OHdG] for NAD\/DM was 130\u2009pg\/mL (IQR25-75: 51.73-157.2), CVSM 125.8\u2009pg\/mL (IQR25-75: 62.8-170.8), EPM 120.6\u2009pg\/mL (IQR25-75: 87.23-229.7), and control 157.6\u2009pg\/mL (IQR25-75: 97.15-245.6). Poisson regression analysis showed no difference established once confounding variables were considered.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Eight-OHdG did not aid in antemortem diagnosis of NAD\/DM in this cohort of horses. At the time of diagnosis horses with NAD\/DM do not have ongoing oxidative stress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.16988","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T06:49:20Z","timestamp":1704955760000},"page":"1207-1213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Measurement of 8-hydroxy-2\u2032-deoxyguanosine in serum and cerebrospinal fluid of horses with neuroaxonal degeneration and other causes of proprioceptive ataxia"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5605-5639","authenticated-orcid":false,"given":"Megan","family":"Palmisano","sequence":"first","affiliation":[{"name":"New Bolton Center, University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, , Kennett Square, Pennsylvania,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeaneen","family":"Kulp","sequence":"additional","affiliation":[{"name":"New Bolton Center, University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, , Kennett Square, Pennsylvania,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Bender","sequence":"additional","affiliation":[{"name":"New Bolton Center, University of Pennsylvania School of Veterinary Medicine Department of Pathobiology, , Kennett Square, Pennsylvania,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4654-0569","authenticated-orcid":false,"given":"Darko","family":"Stefanovski","sequence":"additional","affiliation":[{"name":"New Bolton Center, University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, , Kennett Square, Pennsylvania,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Robinson","sequence":"additional","affiliation":[{"name":"New Bolton Center, University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, , Kennett Square, Pennsylvania,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Johnson","sequence":"additional","affiliation":[{"name":"New Bolton Center, University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, , Kennett Square, Pennsylvania,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,3,1]]},"reference":[{"issue":"2","key":"2026020304071134500_jvim16988-bib-0001","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1177\/0300985812439074","article-title":"Evidence of oxidative injury of the spinal cord in 2 horses with equine degenerative 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