{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:44:14Z","timestamp":1791182654285,"version":"4.1.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":643,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL086350"],"award-info":[{"award-number":["HL086350"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["TR001861"],"award-info":[{"award-number":["TR001861"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL086350"],"award-info":[{"award-number":["HL086350"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["TR001861"],"award-info":[{"award-number":["TR001861"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Glanzmann's thrombasthenia (GT) is a congenital platelet disorder affecting approximately 1:1\u2009000\u2009000 people globally and characterized by impaired platelet aggregation and clot retraction. Autosomal recessive, loss-of-function, variants in ITGA2B or ITGB3 of the \u03b1IIb\u03b23 receptor cause the disease in humans. A cat affected by Glanzmann's and macrothrombocytopenia was presented to the UC Davis VMTH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>Severe thrombopathia in this cat has an underlying genetic etiology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>A single affected patient, 2 age-matched clinically healthy controls, and a geriatric population (n\u2009=\u200920) of normal cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Physical examination and clinical pathology tests were performed on the patient. Flow cytometry and platelet aggregometry analyses for patient phenotyping were performed. Patient and validation cohort gDNA samples were extracted for Sanger sequencing of a previously identified ITGA2B (c.1986delC) variant. Reverse transcriptase PCR was performed on patient and healthy control PRP samples to verify ITGA2B variant consequence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A novel c.1986_1987insCC autosomal recessive variant in ITGA2B was identified. This variant was absent in a population of 194 unrelated cats spanning 44 different breeds. Complete loss of ITGA2B transcript and protein expression was verified by RT-PCR and flow cytometry, explaining the underlying etiology of GT, and likely macrothrombocytopenia, in this cat.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>This study emphasizes the role of precision medicine in cardiovascular disease of cats and identified yet another variant that may be of utility for screening in the feline population. This study provides a small-volume, standardized, successful protocol for adequate platelet RNA isolation and subsequent molecular assessment of gene expression in cats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.17030","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T06:50:29Z","timestamp":1709275829000},"page":"1408-1417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel\n                    <i>ITGA2B<\/i>\n                    double cytosine frameshift variant (c.1986_1987insCC) leads to Glanzmann's thrombasthenia in a cat"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5016-3743","authenticated-orcid":false,"given":"Victor N.","family":"Rivas","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, University of California-Davis Department of Medicine and Epidemiology, , Davis, California,","place":["USA"]},{"name":"College of Veterinary Medicine, North Carolina State University Department of Clinical Sciences, , Raleigh, North Carolina,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avalene W. K.","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California-Davis Department of Surgical and Radiological Sciences, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meg","family":"Shaverdian","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California-Davis Department of Surgical and Radiological Sciences, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nghi P.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California-Davis Department of Surgical and Radiological Sciences, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalena R.","family":"Wouters","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California-Davis Department of Medicine and Epidemiology, , Davis, California,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5611-5745","authenticated-orcid":false,"given":"Joshua A.","family":"Stern","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California-Davis Department of Medicine and Epidemiology, , Davis, California,","place":["USA"]},{"name":"College of Veterinary Medicine, North Carolina State University Department of Clinical Sciences, , Raleigh, North Carolina,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3325-2744","authenticated-orcid":false,"given":"Ronald H. 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