{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:57:22Z","timestamp":1790834242162,"version":"4.1.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":643,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T00:00:00Z","timestamp":1713139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejer\u00eda de Universidad, Investigaci\u00f3n e Innovaci\u00f3n. Junta de Andalucia","award":["Grupo PAIDI AGR-277"],"award-info":[{"award-number":["Grupo PAIDI AGR-277"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The immature platelet fraction (IPF), a parameter obtained by the Sysmex XN-1000V analyzer, is used in humans to differentiate between central (CEN) and peripheral (PER) thrombocytopenia (TP) but has not been evaluated in small animals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Compare IPF between healthy, clinical non-TP and TP dogs and cats, study IPF in different causes of TP in dogs and cats and, establish IPF reference intervals (RIs), and study the effect of age and sex on IPF in healthy dogs and cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>A total of 3281 dogs and 726 cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Retrospective review of medical records. Animals were classified as nonthrombocytopenic (healthy group and group of clinical patients without TP [NTP]) or TP. These latter animals were subclassified as pseudothrombocytopenia (PSE), CEN and PER, based on evaluation of platelet clumps, estimated platelet count in blood smears and final diagnosis. Blood samples were evaluated using a Sysmex XN-1000V with a specific platelet channel (PLT-F).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The IPF was significantly different between each subtype of TP in both species. Immature platelet fractions &amp;lt;6.9% in dogs or 13.6% in cats, once PSE has been eliminated by review of blood smears, are indicative of CEN. Reference intervals for IPF were 0.5%-8% in healthy dogs and 1%-40.3% in healthy cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>We determined that IPF can differentiate between CEN and PER in dogs and cats, guiding additional testing and avoiding more invasive procedures (bone marrow sampling). A blood smear always should be evaluated to rule out platelet clumping.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.17074","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T07:59:38Z","timestamp":1713167978000},"page":"1512-1519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Utility of immature platelet fraction in the Sysmex XN-1000V for the differential diagnosis of central and peripheral thrombocytopenia in dogs and cats"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4086-0940","authenticated-orcid":false,"given":"Alejandro","family":"Perez-Ecija","sequence":"first","affiliation":[{"name":"University of Cordoba, Campus Rabanales Department of Animal Medicine and Surgery, , Cordoba,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Martinez","sequence":"additional","affiliation":[{"name":"University of Cordoba, Campus Rabanales Department of Animal Medicine and Surgery, , Cordoba,","place":["Spain"]},{"name":"Veterinary Teaching Hospital, University of Cordoba, Campus Rabanales , Cordoba,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Fernandez-Casta\u00f1er","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, University of Cordoba, Campus Rabanales , Cordoba,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7725-8080","authenticated-orcid":false,"given":"Francisco J.","family":"Mendoza","sequence":"additional","affiliation":[{"name":"University of Cordoba, Campus Rabanales Department of Animal Medicine and Surgery, , Cordoba,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,5,1]]},"reference":[{"issue":"3","key":"2026020305121071300_jvim17074-bib-0001","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/j.1748-5827.2004.tb00246.x","article-title":"Haematological and biochemical abnormalities in canine blood: frequency and associations in 1022 samples","volume":"45","author":"Comazzi","year":"2004","journal-title":"J Small Anim Pract"},{"issue":"3","key":"2026020305121071300_jvim17074-bib-0002","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1111\/j.1939-165X.2002.tb00274.x","article-title":"Quantitation of reticulated platelets in healthy dogs and in nonthrombocytopenic dogs with clinical disease","volume":"31","author":"Smith","year":"2002","journal-title":"Vet Clin Pathol"},{"key":"2026020305121071300_jvim17074-bib-0003","first-page":"675","article-title":"Schalm's Veterinary Hematology","author":"Wasserkug-Naor","year":"2022","edition":"7th ed."},{"key":"2026020305121071300_jvim17074-bib-0004","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1007\/BF02642508","article-title":"Evaluation of reticulated platelets in dogs","volume":"8","author":"Weiss","year":"1998","journal-title":"Comp Haematol Int"},{"issue":"3","key":"2026020305121071300_jvim17074-bib-0005","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1309\/AJCPUAGGB4URL5XO","article-title":"New fluorescent method (PLT-F) on Sysmex XN2000 hematology analyzer achieved higher accuracy in low platelet counting","volume":"140","author":"Schoorl","year":"2013","journal-title":"Am J Clin Pathol"},{"issue":"3","key":"2026020305121071300_jvim17074-bib-0006","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1111\/vcp.12936","article-title":"Validation of the Sysmex XN-V hematology analyzer for canine specimens","volume":"50","author":"Grebert","year":"2021","journal-title":"Vet Clin Pathol"},{"issue":"3","key":"2026020305121071300_jvim17074-bib-0007","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s12185-015-1741-0","article-title":"Clinical significance of IPF% or RP% measurement in distinguishing primary immune thrombocytopenia from aplastic thrombocytopenic disorders","volume":"101","author":"Sakuragi","year":"2015","journal-title":"Int J Hematol"},{"issue":"3","key":"2026020305121071300_jvim17074-bib-0008","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/j.1600-0609.2010.01468.x","article-title":"Correlation between immature platelet fraction and reticulated platelets. 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