{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:04:29Z","timestamp":1787321069276,"version":"build-2736575974"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":459,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016749","name":"UC Davis Center for Equine Health","doi-asserted-by":"publisher","award":["21-Q"],"award-info":[{"award-number":["21-Q"]}],"id":[{"id":"10.13039\/100016749","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016749","name":"UC Davis Center for Equine Health","doi-asserted-by":"publisher","award":["21\u2010Q"],"award-info":[{"award-number":["21\u2010Q"]}],"id":[{"id":"10.13039\/100016749","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Furosemide, a commonly used diuretic, activates the renin-angiotensin-aldosterone system (RAAS) in other species. Little is known about RAAS peptide activation in horses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>To evaluate equilibrium analysis as a practical method for RAAS quantification in horses and describe the RAAS response to a single dose of furosemide. We hypothesize that furosemide would cause transient increase in RAAS peptides in horses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>14 healthy adult thoroughbreds from a university teaching herd.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Horses received either furosemide (1\u2009mg\/kg IV) or saline IV in a crossover study design. Protease-inhibited samples were compared with equilibrium analysis samples with Deming regression analysis. Renin-angiotensin-aldosterone system hormones were evaluated at 0, 0.25, 0.5, 4, and 24\u2009hours postadministration, via equilibrium analysis. Values were compared with a mixed effects model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Correlation between protease inhibition and equilibrium analysis was high for angiotensin I peptide (AngI) and angiotensin II peptide (AngII) (r\u2009=\u2009.92 and .95, respectively). Baseline RAAS peptide concentrations were below the limit of detection except AngII (median, 7.5 [range, 3.5-14.0] pmol\/L). Furosemide administration resulted in an increase in AngI (8.0 [0.5-15.5] pmol\/L, P\u2009=\u2009.03), AngII (33.7 [9.6-57.9] pmol\/L, P\u2009=\u2009.0008), angiotensin III peptide (AngIII) (2.9 [0.9-4.9] pmol\/L, P\u2009=\u2009.0005), angiotensin IV peptide (AngIV) (2.0 [0.6-3.4] pmol\/L, P\u2009=\u2009.0005), and angiotensin 1-5 peptide (Ang1-5) (5.6 [1.2-5.9] pmol\/L, P\u2009=\u2009.003) at 4\u2009hours. Differences are reported as difference in the mean (95% confidence interval [CI]).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Furosemide produced an increase in hormones associated with both the classical and alternative RAAS pathways. Serum equilibrium analysis is practical for RAAS analysis in horses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.17208","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T03:00:48Z","timestamp":1729566048000},"page":"3272-3280","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of furosemide on comprehensive renin-angiotensin-aldosterone system activity of Thoroughbred horses"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7999-8704","authenticated-orcid":false,"given":"Mallory L.","family":"Lehman","sequence":"first","affiliation":[{"name":"Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California-Davis , Davis, 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