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Homogenized tissues were incubated with spiked angiotensin I or II (AngII) under control and inhibitor conditions to assess relative enzyme contributions to AngII or angiotensin 1\u20137 formation. Freezing was delayed (2- or 7-h in Groups 1 and 2, respectively) in paired samples, to evaluate the effect of time to freezing on RAS enzyme activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Samples were harvested within 20\u2009min of euthanasia (Group 1) or a median of 21.9\u2009h after death or euthanasia (Group 2). RAS enzymes were active in all samples. The median contribution of angiotensin-converting enzyme to AngII formation was greater than 88% in all tissues. The 90% confidence limits of the geometric mean of the ratio of the angiotensin production of paired samples in 10\/14 (Group 1) and 16\/19 (Group 2) experiments met equivalence requirements (lower bound &amp;gt;\u20090.8 and upper bound &amp;lt;\u20091.2).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Renin\u2013angiotensin system enzymes are present and active in canine myocardial and renal tissues for greater than 24\u2009h postmortem.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.70181","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T15:19:22Z","timestamp":1751469562000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Myocardial and Renal Renin\u2013Angiotensin System Enzyme Activity in 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