{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T23:33:10Z","timestamp":1775691190354,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T00:00:00Z","timestamp":1416528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Arag\u00f3n Government"},{"name":"INIA","award":["RTA2011-00103-00-00"],"award-info":[{"award-number":["RTA2011-00103-00-00"]}]},{"name":"MICIN","award":["AGL2011-30453-C04"],"award-info":[{"award-number":["AGL2011-30453-C04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pharm &amp; Therapeutics"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>The aim of this work was developing effective treatments against<jats:italic>Brucella suis<\/jats:italic>biovar 2, responsible for swine brucellosis in Europe.<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s for antibiotics used classically in brucellosis and two new macrolides (tulathromycin and tildipirosin) were determined for 33<jats:italic>B.\u00a0suis<\/jats:italic>biovar 2 field and<jats:italic>B.\u00a0suis<\/jats:italic>reference strains.<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content><jats:sub>90<\/jats:sub>values ranged from 0.01 to 0.25\u00a0\u03bcg\/mL. The best candidates, given alone or combined, were then evaluated in mice. Ten groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a07) of<jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were inoculated (1\u00a0\u00d7\u00a010<jats:sup>5<\/jats:sup><jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/mouse) with a virulent<jats:italic>B.\u00a0suis<\/jats:italic>biovar 2 field strain. All groups, excepting untreated control, were treated for 14 days with, respectively, doxycycline, dihydrostreptomycin, tulathromycin (one or two doses), or tildipirosin (one or two doses) given alone, and doxycycline combined with dihydrostreptomycin, tulathromycin, or tildipirosin. Combined tildipirosin treatment was the most effective, then selected for pig studies. Sixteen<jats:italic>B. suis<\/jats:italic>biovar 2 naturally infected sows were treated with oxytetracycline (20\u00a0mg\/kg<jats:styled-content style=\"fixed-case\">BW<\/jats:styled-content>\/daily) for 21\u00a0days. The half of these received also tildipirosin (4\u00a0mg\/kg<jats:styled-content style=\"fixed-case\">BW<\/jats:styled-content>) in two doses with a 10\u2010day interval. An extensive bacteriological study conducted ten days after ceasing treatments proved the efficacy of this combined oxytetracycline\/tildipirosin treatment.<\/jats:p>","DOI":"10.1111\/jvp.12189","type":"journal-article","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T05:29:29Z","timestamp":1416547769000},"page":"357-364","source":"Crossref","is-referenced-by-count":7,"title":["Studies on a suitable antibiotic therapy for treating swine brucellosis"],"prefix":"10.1111","volume":"38","author":[{"given":"L.","family":"Dieste\u2010P\u00e9rez","sequence":"first","affiliation":[{"name":"Unidad de Sanidad Animal Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria (CITA) Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Fraile","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal Universitat de Lleida Lleida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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