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Minimum inhibitory concentrations (MIC) and phenicol resistance genes were determined for 169<jats:italic>S.\u00a0pseudintermedius<\/jats:italic>and 167<jats:italic>E.\u00a0coli<\/jats:italic>isolates. Minimum bactericidal concentrations (MBC), time\u2010killing kinetics, and postantibiotic effect (PAE) of both agents against wild\u2010type isolates of each species were assessed. For<jats:italic>S.\u00a0pseudintermedius,<\/jats:italic>the chloramphenicol MIC<jats:sub>90<\/jats:sub>was 32\u00a0\u03bcg\/mL. No florfenicol resistance was detected in this species (MIC<jats:sub>90<\/jats:sub>\u00a0<jats:sub>=<\/jats:sub>\u00a04\u00a0\u03bcg\/mL). The MIC<jats:sub>90<\/jats:sub>of both agents against<jats:italic>E.\u00a0coli<\/jats:italic>was 8\u00a0\u03bcg\/mL. Resistance genes found were<jats:italic>cat<\/jats:italic><jats:sub>pC221<\/jats:sub>in<jats:italic>S.\u00a0pseudintermedius<\/jats:italic>and<jats:italic>catA1<\/jats:italic>and\/or<jats:italic>floR<\/jats:italic>in<jats:italic>E.\u00a0coli<\/jats:italic>. The phenicols displayed a time\u2010dependent, mainly, bacteriostatic effect on both species. Prolonged PAEs were observed for<jats:italic>S.\u00a0pseudintermedius<\/jats:italic>, and no PAEs were detected for<jats:italic>E.\u00a0coli<\/jats:italic>. More research into determination of PK\/PD targets of efficacy is needed to further assess the clinical use of chloramphenicol and florfenicol as second\u2010line agents in dogs, optimize dosage regimens, and set up species\u2010specific clinical break points.<\/jats:p>","DOI":"10.1111\/jvp.12204","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T06:11:14Z","timestamp":1422339074000},"page":"443-450","source":"Crossref","is-referenced-by-count":13,"title":["<i>In vitro<\/i>assessment of chloramphenicol and florfenicol as second\u2010line antimicrobial agents in dogs"],"prefix":"10.1111","volume":"38","author":[{"given":"M. G.","family":"Maaland","sequence":"first","affiliation":[{"name":"Department of Veterinary Disease Biology Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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