{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:36:57Z","timestamp":1790267817063,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,1,17]],"date-time":"2017-01-17T00:00:00Z","timestamp":1484611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007588","name":"Washington State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007588","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pharm &amp; Therapeutics"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p>P\u2010glycoprotein (P\u2010gp), encoded by the <jats:styled-content style=\"fixed-case\">ABCB<\/jats:styled-content>1 (<jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content>1) gene, dramatically impacts drug disposition. P\u2010gp is expressed in the intestines, biliary canaliculi, renal tubules, and brain capillaries where it functions to efflux substrate drugs. In this capacity, P\u2010gp restricts oral absorption, enhances biliary and renal excretion, and inhibits central nervous system entry of substrate drugs. Many drugs commonly used in veterinary medicine are known substrates for canine P\u2010gp (vincristine, loperamide, ivermectin, others). Because these drugs have a narrow therapeutic index, defective P\u2010gp function can cause serious adverse drug reactions due to enhanced brain penetration and\/or decreased clearance. P\u2010gp dysfunction in dogs can be intrinsic (dogs harboring <jats:styled-content style=\"fixed-case\">ABCB<\/jats:styled-content>1\u20101\u0394) or acquired (drug interactions between a P\u2010gp inhibitor and P\u2010gp substrate). New human drug candidates are required to undergo assessment for P\u2010gp interactions according to <jats:styled-content style=\"fixed-case\">FDA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> regulations to avoid adverse drug reactions and drug\u2013drug interactions. Similar information regarding canine P\u2010gp could prevent adverse drug reactions in dogs. Because differences in P\u2010gp substrates have been documented between species, one should not presume that human or murine P\u2010gp substrates are necessarily canine P\u2010gp substrates. Thus, our goal was to develop a cell line for assessing drugs as canine P\u2010gp substrates.<\/jats:p>","DOI":"10.1111\/jvp.12390","type":"journal-article","created":{"date-parts":[[2017,1,17]],"date-time":"2017-01-17T09:50:17Z","timestamp":1484646617000},"page":"545-551","source":"Crossref","is-referenced-by-count":18,"title":["Establishment of a cell line for assessing drugs as canine P\u2010glycoprotein substrates: proof of principle"],"prefix":"10.1111","volume":"40","author":[{"given":"K. 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