{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:53:06Z","timestamp":1790358786123,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T00:00:00Z","timestamp":1493856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009082","name":"Universidad Cat\u00f3lica de C\u00f3rdoba","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009082","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002911","name":"Universidad Complutense de Madrid","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002911","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pharm & Therapeutics"],"published-print":{"date-parts":[[2017,12]]},"abstract":"The main objectives of this study were (i) to evaluate the serum pharmacokinetic behaviour and milk penetration of marbofloxacin (MFX; 5\u00a0mg\/kg), after intravenous (IV<\/jats:styled-content>) and intramuscular (IM<\/jats:styled-content>) administration in lactating goats and simulate a multidose regimen on steady\u2010state conditions, (ii) to determine the minimum inhibitory concentration (MIC<\/jats:styled-content>) and mutant prevention concentration (MPC<\/jats:styled-content>) of coagulase negative staphylococci (CNS<\/jats:styled-content>) isolated from caprine mastitis in C\u00f3rdoba, Argentina and (iii) to make a PK<\/jats:styled-content>\/PD<\/jats:styled-content> analysis by Monte Carlo simulation from steady\u2010state pharmacokinetic parameters of MFX<\/jats:styled-content> by IV<\/jats:styled-content> and IM<\/jats:styled-content> routes to evaluate the efficacy and risk of the emergence of resistance. The study was carried out with six healthy, female, adult Anglo Nubian lactating goats. Marbofloxacin was administered at 5\u00a0mg\/kg bw by IV<\/jats:styled-content> and IM<\/jats:styled-content> route. Serum and milk concentrations of MFX were determined with HPLC<\/jats:styled-content>\/uv. From 106 regional strains of CNS<\/jats:styled-content> isolated from caprine mastitis in herds from C\u00f3rdoba, Argentina, MIC<\/jats:styled-content>s and MPC<\/jats:styled-content>s were determined. MIC<\/jats:styled-content>90<\/jats:sub> and MPC<\/jats:styled-content>90<\/jats:sub> were 0.4 and 6.4\u00a0\u03bcg\/ml, respectively. MIC<\/jats:styled-content> and MPC<\/jats:styled-content>\u2010based PK<\/jats:styled-content>\/PD<\/jats:styled-content> analysis by Monte Carlo simulation indicates that IV<\/jats:styled-content> and IM<\/jats:styled-content> administration of MFX<\/jats:styled-content> in lactating goats may not be adequate to recommend it as an empirical therapy against CNS<\/jats:styled-content>, because the most exigent endpoints were not reached. Moreover, this dose regimen could increase the probability of selecting mutants and resulting in emergence of resistance. Based on the results of Monte Carlo simulation, the optimal dose of MFX<\/jats:styled-content> to achieve an adequate antimicrobial efficacy should be 10\u00a0mg\/kg, but it is important take into account that fluoroquinolones are substrates of efflux pumps, and this fact may determine that assumption of linear pharmacokinetics at high doses of MFX<\/jats:styled-content> may be incorrect.<\/jats:p>","DOI":"10.1111\/jvp.12409","type":"journal-article","created":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T07:32:40Z","timestamp":1493883160000},"page":"629-640","source":"Crossref","is-referenced-by-count":15,"title":["Pharmacokinetics, milk penetration and PK<\/scp>\/PD<\/scp> analysis by Monte Carlo simulation of marbofloxacin, after intravenous and intramuscular administration to lactating goats"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9799-4499","authenticated-orcid":false,"given":"A. 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P.","family":"Zarazaga","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agropecuarias\u2010Unidad Asociada al CONICET Universidad Cat\u00f3lica de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. I.","family":"San Andr\u00e9s","sequence":"additional","affiliation":[{"name":"Departamento de Toxicolog\u00eda y Farmacolog\u00eda Facultad de Veterinaria Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"De Lucas","sequence":"additional","affiliation":[{"name":"Departamento de Toxicolog\u00eda y Farmacolog\u00eda Facultad de Veterinaria Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020243615928"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.1979"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.03.011"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(00)00185-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2793-2797.2001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2015.02.001"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01038.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2003030"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2013.04.023"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00759"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.11.025"},{"key":"e_1_2_6_13_1","volume-title":"Performance standards for antimicrobial susceptibility testing; twenty\u2010third informational supplement. 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