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Adverse effects of <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content> have previously been demonstrated in some species, but so far there have been no studies looking specifically at the impact of <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content> on pigs. In this study, three different doses of <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content> (5, 25 and 125\u00a0mg\u00a0kg\u00a0bw<jats:sup>\u22121<\/jats:sup>) were administrated to Bama pigs. The results showed that lipid peroxidation of pig liver tissue occurred with all <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content> doses. The 125\u00a0mg\u00a0kg dose of <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content> induced catalase (<jats:styled-content style=\"fixed-case\">CAT<\/jats:styled-content>) and glutathione peroxidase (<jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content>\u2010px) and increased <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>450 content in pig liver microsomes. The activity of microsomal <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content>\u2010cytochrome C reductase (<jats:styled-content style=\"fixed-case\">NCCR<\/jats:styled-content>) was elevated at both the 25 and 125\u00a0mg\u00a0kg doses of <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>. Microsomal erythromycin N\u2010demethylase (<jats:styled-content style=\"fixed-case\">ERND<\/jats:styled-content>) and aminopyrin N\u2010demethylase (<jats:styled-content style=\"fixed-case\">AND<\/jats:styled-content>) were inhibited by high doses of <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>, while aniline\u20104\u2010hydroxylase (<jats:styled-content style=\"fixed-case\">AH<\/jats:styled-content>) was unaffected. None of the <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content> treatments affected superoxide dismutase (<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>) or cytochrome b5 content. Antioxidases and microsomal enzymes may work together to resist the adverse effects of <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>. Proteomic analysis revealed increased protein expression of carboxylesterase (<jats:styled-content style=\"fixed-case\">CES<\/jats:styled-content>) and alpha\u2010enolase (<jats:styled-content style=\"fixed-case\">ENO<\/jats:styled-content>1) in microsomes as a stress response to <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>. These results provide new information about the adverse effect of fluoroquinolones and help guide their usage more effectively in the clinic or animal breeding.<\/jats:p>","DOI":"10.1111\/jvp.12493","type":"journal-article","created":{"date-parts":[[2018,4,14]],"date-time":"2018-04-14T07:08:37Z","timestamp":1523689717000},"page":"562-571","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of enrofloxacin on antioxidant system, microsomal enzymatic activity, and proteomics in porcine liver"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2629-7941","authenticated-orcid":false,"given":"Y.","family":"Li","sequence":"first","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Mou","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Thunders","sequence":"additional","affiliation":[{"name":"College of Health Massey University  Wellington New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Ai","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Qiu","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.5958\/2277-8934.2014.00029.0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.115.064717"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_9_5_1","unstructured":"Cao Z. 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