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The pharmacological support includes anti\u2010inflammatory, antimicrobials, and antimycotic drugs, but therapeutic failure and antimicrobial resistance are leading to alternative strategies based on phytotherapic products. This study aimed to evaluate an essential oil blend (Otogen<jats:sup>\u00ae<\/jats:sup>) to treat otitis externa in dogs. The experimental design was divided in: (a) an <jats:italic>in vitro<\/jats:italic> approach, based on the European Normative UNI EN 1275:2006, to assess the efficacy of the product against the most frequently isolated microorganisms during otitis externa. (b) an <jats:italic>in vivo<\/jats:italic> part, 12 owned dogs presenting with acute otitis externa were enrolled. A significant growth reduction (&gt;99.9%) of <jats:italic>Malassezia pachydermatis<\/jats:italic> and <jats:italic>Candida albicans<\/jats:italic> after 15\u00a0min of contact and <jats:italic>Pseudomonas aeruginosa<\/jats:italic> after 1\u00a0h of incubation was recorded. For <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>, 50% of growth reduction were appreciated after 15\u00a0min. Results obtained <jats:italic>in vivo<\/jats:italic> after 7\u00a0days of blend administration, noted a significant improvement of all the considered parameters (most important were head shaking, erythema, and scraping). The results obtained may support the usefulness of the tested phytotherapic blend to manage acute otitis externa in dogs.<\/jats:p>","DOI":"10.1111\/jvp.13000","type":"journal-article","created":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T06:40:28Z","timestamp":1626244828000},"page":"910-918","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["In vitro and in vivo evaluation of a new phytotherapic blend to treat acute externa otitis in dogs"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7766-4647","authenticated-orcid":false,"given":"Cristina","family":"Vercelli","sequence":"first","affiliation":[{"name":"Department of Veterinary Science of Turin University of Turin Turin 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