{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:34:14Z","timestamp":1782920054093,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Pharm &amp; Therapeutics"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A pharmacokinetic\/pharmacodynamic (PK\/PD) model was developed to optimize the dosing regimen of enrofloxacin (EN) against<jats:italic>Glaesserella parasuis<\/jats:italic>in pigs. EN (2.5\u00a0mg\/kg) was administered intramuscularly to eight healthy pigs and eight pigs that were experimentally infected with<jats:italic>G<\/jats:italic>.\u00a0<jats:italic>parasuis<\/jats:italic>SW124. Blood samples were collected at predetermined time points. Plasma EN concentrations were determined, and the main PK parameters were estimated. The PD of EN against<jats:italic>G<\/jats:italic>.\u00a0<jats:italic>parasuis<\/jats:italic>SW124 was also investigated in vitro and ex vivo. The dynamic behaviour of EN in pigs was consistent with a one\u2010compartment model. Significant differences were observed between healthy and infected pigs in the area under the curve (AUC) (3.58\u00a0\u00b1\u00a00.94 and 5.39\u00a0\u00b1\u00a01.01\u00a0\u03bcg\u00a0h\/ml, respectively) and the systemic clearance (CL) (736.32\u00a0\u00b1\u00a0171.46 and 479.36\u00a0\u00b1\u00a096.81\u00a0ml\/h\/kg, respectively), suggesting that the pathogenicity of<jats:italic>G<\/jats:italic>.\u00a0<jats:italic>parasuis<\/jats:italic>SW124 to pigs might alter the PK profile of EN, and therefore should be considered in dose optimization. Both the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 0.125\u00a0\u03bcg\/ml in tryptone soya broth (TSB) medium or plasma. The mutant prevention concentration (MPC) was 0.6\u00a0\u03bcg\/ml. EN inhibited or killed<jats:italic>G<\/jats:italic>.<jats:italic>parasuis<\/jats:italic>SW124 in a concentration\u2010dependent manner. The targeted endpoints of AUC<jats:sub>24\u00a0h<\/jats:sub>\/MIC for bacteriostasis, bactericidal action, and eradication were 5.10, 7.34, and 8.65\u00a0h and 5.91, 9.01, and 10.90\u00a0h in healthy and infected pigs, respectively. The optimal doses were 3.58\u22126.08\u00a0mg\/kg in healthy pigs and 2.71\u22124.99\u00a0mg\/kg in infected pigs from the point of view of preventing drug resistance.<\/jats:p>","DOI":"10.1111\/jvp.13055","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T12:19:47Z","timestamp":1648556387000},"page":"291-300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["PK\/PD modelling of enrofloxacin against<i>Glaesserella parasuis<\/i>infection in pigs"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3052-1814","authenticated-orcid":false,"given":"Bo","family":"Yang","sequence":"first","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao Dong","family":"Li","sequence":"additional","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Chen","sequence":"additional","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Hong","sequence":"additional","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Liu","sequence":"additional","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Ping","family":"Zheng","sequence":"additional","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng Yang","family":"Ou","sequence":"additional","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6733-5169","authenticated-orcid":false,"given":"Dao Jin","family":"Yu","sequence":"additional","affiliation":[{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province\/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.02.012"},{"key":"e_1_2_10_3_1","volume-title":"Isolation and characterization of Haemophilus parasuis and development of its diagnostic method and inactivated bacterin","author":"Cai X. 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