{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T10:38:48Z","timestamp":1780396728915,"version":"3.54.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Pharm &amp; Therapeutics"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Uterine vascular alterations take place in pyometra bitches speculatively influenced by prostaglandin and nitric oxide pathways. However, no causative effect of nitric oxide on endometrial vascularization was proved elsewhere for medically treated pyometra bitches. This study aimed to identify the main in\u00a0situ uterine artery vasodilation pathway in pyometra bitches medically treated with antigestagen solely or coupled with prostaglandin. Pyometra bitches were enrolled into groups: Ovariohysterectomy at diagnosis (Control\u2010OHE; <jats:italic>n<\/jats:italic>\u2009=\u20097), Antigestagen (10\u2009mg\/kg aglepristone on Days 1, 2, and 8 after diagnosis; <jats:italic>n<\/jats:italic>\u2009=\u20095), and Antigestagen\u2009+\u2009luteolytic (aglepristone plus 1\u2009\u03bcg\/kg of cloprostenol from Days 1\u20137; <jats:italic>n<\/jats:italic>\u2009=\u20095). Treated bitches were ovariohysterectomized after 8\u2009days of treatment. Uterine artery fragments from all bitches were collected for tissue nitric oxide and prostaglandin E<jats:sub>2<\/jats:sub> assays. Control\u2010OHE group had lower uterine artery concentration of nitric oxide compared to treated bitches (Antigestagen and Antigestagen\u2009+\u2009luteolytic groups). No significant difference was verified between the medical treated groups. Uterine artery concentration of prostaglandin E<jats:sub>2<\/jats:sub> was not different between control and treated bitches, as well as between both treated groups. In conclusion, nitric oxide and prostaglandin E<jats:sub>2<\/jats:sub> are not directly involved in vascular modulation of the uterine artery, albeit pyometra medical treatment influences nitric oxide concentration in the uterine artery.<\/jats:p>","DOI":"10.1111\/jvp.13482","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T18:33:17Z","timestamp":1726597997000},"page":"22-29","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Situ Uterine Artery Prostaglandin <scp>E<sub>2<\/sub><\/scp> and Nitric Oxide in Open\u2010Cervix Pyometra and Medically Treated Bitches"],"prefix":"10.1111","volume":"48","author":[{"given":"Tha\u00eds Gomes","family":"Faustino","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Rodrigues","family":"da Rosa Filho","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Claudia Pereda","family":"Francischini","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u00edra Morales","family":"Brito","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Souza Ramos","family":"Angrimani","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8198-2223","authenticated-orcid":false,"given":"Camila Infantosi","family":"Vannucchi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,17]]},"reference":[{"issue":"7","key":"e_1_2_10_2_1","first-page":"340","article-title":"Canine Pyometra","volume":"3","author":"Baithalu R. 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