{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:37:26Z","timestamp":1773283046782,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015226","name":"Royal Veterinary College","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Pharm &amp; Therapeutics"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study examines the pharmacodynamics (PD) of florfenicol (FFN) for treating porcine respiratory diseases by comparing its effects on <jats:italic>Glaesserella parasuis<\/jats:italic>, <jats:styled-content style=\"fixed-case\"><jats:italic>Actinobacillus pleuropneumoniae<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Pasteurella multocida<\/jats:italic><\/jats:styled-content>. In\u00a0vitro time\u2010kill assays and semi\u2010mechanistic PD modeling were used to assess bacterial growth and killing rates at varying FFN concentrations. Species\u2010specific PD models indicated that <jats:italic>f<\/jats:italic>AUC\/MIC was the best PK\/PD index across all species. <jats:styled-content style=\"fixed-case\"><jats:italic>A. pleuropneumoniae<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>P. multocida<\/jats:italic><\/jats:styled-content> had target values of 1.05 and 1.66\u2009\u00d7\u2009MIC, respectively for bacteriostasis and 1.12 and 1.87\u2009\u00d7\u2009MIC for 99.9% kill. Two phenotypes of <jats:italic>G. parasuis<\/jats:italic> emerged \u201cfast\u2010kill\u201d and \u201cslow\u2010kill\u201d which displayed distinct bacterial eradication rates despite similar MICs. For \u201cslow\u2010kill\u201d isolates, an average free drug concentration (<jats:italic>f<\/jats:italic>AUC\/MIC) of 1.46 and 1.63\u2009\u00d7\u2009MIC (median, range: 1.53\u20131.69) was required for bacteriostasis and 99.9% kill. \u201cFast\u2010kill\u201d isolates needed an average free drug concentration of 1.38\u2009\u00d7\u2009MIC for bacteriostasis and 1.51\u2009\u00d7\u2009MIC for a 99.9% reduction. Indicating that the rate of kill influences the respective average free concentration required to achieve an equivalent antibacterial effect. Simulations of clinical dosing of FFN predicted bacterial eradication for all species, highlighting the value of phenotype\u2010specific PD modeling in guiding treatment strategies for porcine respiratory infections.<\/jats:p>","DOI":"10.1111\/jvp.13500","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T06:25:27Z","timestamp":1738909527000},"page":"318-339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phenotype\u2010Specific Semi\u2010Mechanistic Modelling of Florfenicol Time\u2010Kill Curves in <i>G. Parasuis<\/i> Compared to Other Respiratory Pathogens"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3550-3030","authenticated-orcid":false,"given":"Andrew","family":"Mead","sequence":"first","affiliation":[{"name":"Comparative Biomedical Sciences The Royal Veterinary College  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail","family":"Hughes","sequence":"additional","affiliation":[{"name":"Comparative Biomedical Sciences The Royal Veterinary College  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Azzariti","sequence":"additional","affiliation":[{"name":"Comparative Biomedical Sciences The Royal Veterinary College  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8846-8892","authenticated-orcid":false,"given":"Pierre\u2010Louis","family":"Toutain","sequence":"additional","affiliation":[{"name":"Comparative 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