{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T11:34:53Z","timestamp":1762774493656,"version":"build-2065373602"},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences","doi-asserted-by":"publisher","award":["YFI20240404","2023TD47"],"award-info":[{"award-number":["YFI20240404","2023TD47"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32173022"],"award-info":[{"award-number":["32173022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFD0901701"],"award-info":[{"award-number":["2019YFD0901701"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Pharm &amp; Therapeutics"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study was conducted to establish population pharmacokinetics (PPK) of florfenicol (FLO) in crayfish (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Procambarus clarkii<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) after a single oral administration at a dose of 15\u2009mg\/kg at 25\u00b0C based on a nonlinear mixed effect model. The sparse sampling method was used to collect the blood samples. Thirty\u2010two crayfish were divided into four groups, and one group included four male crayfish and four female crayfish. One animal undertook three sampling time points. All samples were quantified using high\u2010performance liquid chromatography with an ultraviolet detector. The initial pharmacokinetic (PK) parameters were estimated by reference search and the calculation of a na\u00efve pooled approach. The additive error model was selected using the tool of maximum likelihood model comparison. The covariate model included the two variations of body weight and sex. Through the addition and subtraction of parameters, weight and gender had no significant effect on the alterations of PK parameters. Afterward, the random effects were introduced in the model, which notably reduced the coefficient of variation. Finally, the calculated values of the absorption rate constant, apparent distribution volume, and total systemic clearance were estimated to be 1.93\/h, 11.16\u2009L\/kg, and 2.35\u2009L\/h\/kg, respectively. The secondary parameters of the elimination rate constant, elimination half\u2010life, and area under the concentration\u2010time curve were calculated to be 0.20\/h, 3.47\u2009h, and 6.38\u2009h.mg\/L, respectively. This study supported a concise method for conducting PK studies in crustacean animals that facilitated the development of PK methodology in aquaculture.\n                  <\/jats:p>","DOI":"10.1111\/jvp.70011","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T11:59:27Z","timestamp":1752494367000},"page":"534-540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Population Pharmacokinetics of Florfenicol in Crayfish (\n                    <scp>\n                      <i>Procambarus clarkii<\/i>\n                    <\/scp>\n                    ) Based on the Sparse Sampling Method and a Nonlinear Mixed\u2010Effect Model"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2563-5216","authenticated-orcid":false,"given":"Ning","family":"Xu","sequence":"first","affiliation":[{"name":"Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Tian","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binbin","family":"Gong","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering &amp; Biotechnology XingTai University  Xingtai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongzhen","family":"Ding","sequence":"additional","affiliation":[{"name":"Agro\u2010Environmental Protection Institute Ministry of Agriculture and Rural Affairs  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Ai","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"volume-title":"China Fishery Statistics Yearbook","year":"2024","author":"CFFA","key":"e_1_2_12_2_1"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aqrep.2020.100405"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.16213\/j.cnki.scjas.2013.03.090"},{"issue":"5","key":"e_1_2_12_5_1","first-page":"48","article-title":"Sensitivity Determination and Analysis of Pathogenic Enterobacter cloacae Isolated From Macrobrachium rosenbergii","volume":"48","author":"Li X.","year":"2018","journal-title":"Freshwater Fisheries"},{"issue":"4","key":"e_1_2_12_6_1","first-page":"19","article-title":"Pharmacodynamics of Florfenicol on Bacterium Yersinia ruckeri In\u00a0Vitro","volume":"25","author":"Lu T.","year":"2012","journal-title":"Chinese Journal of Fisheries"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10070416"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102885.2007.00809.x"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14112440"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12562\u2010023\u201001682\u2010w"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.12.081"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695\u2010023\u201001179\u20104"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvp.12672"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.01.037"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.11946\/jfc.20171011013"}],"container-title":["Journal of Veterinary Pharmacology and Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jvp.70011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T11:32:21Z","timestamp":1762774341000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jvp.70011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,14]]},"references-count":14,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/jvp.70011"],"URL":"https:\/\/doi.org\/10.1111\/jvp.70011","archive":["Portico"],"relation":{},"ISSN":["0140-7783","1365-2885"],"issn-type":[{"type":"print","value":"0140-7783"},{"type":"electronic","value":"1365-2885"}],"subject":[],"published":{"date-parts":[[2025,7,14]]},"assertion":[{"value":"2025-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}