{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T02:00:03Z","timestamp":1784944803728,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:00:00Z","timestamp":1768003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:00:00Z","timestamp":1768003200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Pharm &amp;amp; Therapeutics"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diazepam (DZP) residues in aquaculture environments pose a serious threat to food safety. To investigate the pharmacokinetics and residue elimination patterns of DZP using zebrafish as a model organism, a pharmacokinetic study, zebrafish were exposed to 1\u2009mg\/L DZP via bath for 24\u2009h. DZP was metabolized to nordiazepam (NDZP), oxazepam (OZP), and temazepam (TZP). Non\u2010compartmental analysis yielded the following pharmacokinetic parameters:\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1\/2<\/jats:sub>\n                    \u2009=\u20094.25\u201311.78\u2009h; MRT\u2009=\u20093.52\u20137\u2009h; AUC\u2009=\u200934.51\u20134222.69\u2009h\u00b7\u03bcg\/kg; Vd\u2009=\u20091.16\u20136.15\u2009L\/kg; CL\u2009=\u20090.14\u20130.57\u2009L\/h\/kg. The results indicate that DZP and its metabolites are rapidly absorbed and widely distributed in zebrafish, but eliminated slowly. For the residue elimination study, OZP fell below the LOD (0.05\u2009\u03bcg\/kg) after 40\u2009days; NDZP and TZP were undetectable after 60\u2009days. DZP persisted at 0.54\u2009\u00b1\u20090.2\u2009\u03bcg\/kg on day 150, confirming exceptionally slow elimination. The findings are expected to fill a critical data gap concerning the pharmacokinetics and residue elimination of DZP in zebrafish. These findings provide a framework for extending the results to similar rare edible small fish, thereby aiding in food safety assessment and risk management.\n                  <\/jats:p>","DOI":"10.1111\/jvp.70044","type":"journal-article","created":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T09:05:45Z","timestamp":1768035945000},"page":"324-332","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pharmacokinetics and Residue Elimination of Diazepam in Zebrafish"],"prefix":"10.1111","volume":"49","author":[{"given":"Hong\u2010Liang","family":"Xiao","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics, Development and Safety Evaluation South China Agricultural University  Guangzhou P.R. 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