{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:56:04Z","timestamp":1791284164035,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Pharm &amp;amp; Therapeutics"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Penicillin G has been one of the main violative residues detected at slaughter for 25+ years. Accordingly, a residue depletion study was initiated to determine possible reasons, using a two\u2010phase approach. Phase 1 involved a 2 \u00d7\u20092 matrix using either the 4\u2010 or 14\u2010day withdrawal period product at label dosage (1\u00d7) or three times the label dose (3\u00d7), with tissues collected by laparoscopy. Phase 2 employed just a 3\u00d7 dose of both products, with tissues collected at necropsy. Plasma and muscle penicillin G levels were below 50\u2009ng\/g (official tolerance level: 0.050\u2009ppm) before the 4 and 14\u2010day withdrawal period when dosed at 1\u00d7 and 3\u00d7. Penicillin G in kidney and liver samples from heifers administered the 14\u2010day withdrawal product were always below 50\u2009ng\/g by Day 14 regardless of dose or means of tissue collection. Kidney and liver concentration of Pen G from laparoscopically collected samples were in close agreement with results obtained from whole organ analyses. Administration of the 1\u00d7 dose for the 4\u2010day withdrawal period product resulted in residues in kidneys above 50\u2009ng\/g in 25% of study heifers (2 of 8) and in 50% (4 of 8) or more (3 of 5) following a 3\u00d7 label dose.<\/jats:p>","DOI":"10.1111\/jvp.70115","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:40:15Z","timestamp":1791283215000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tissue Depletion in Dairy Cattle Following Administration of Label or Extralabel Doses of Penicillin G Using Two Different Formulations"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7729-6028","authenticated-orcid":false,"given":"Michael J.","family":"Myers","sequence":"first","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine U.S. Food and Drug Administration  Laurel Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1429-0400","authenticated-orcid":false,"given":"O. Alberto","family":"Chiesa","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine U.S. Food and Drug Administration  Laurel Maryland USA"},{"name":"Retired"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9929-0436","authenticated-orcid":false,"given":"Linge","family":"Li","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine U.S. Food and Drug Administration  Laurel Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karyn D.","family":"Howard","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine U.S. Food and Drug Administration  Laurel Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Cohen","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine U.S. Food and Drug Administration  Laurel Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raoul","family":"Gonzales","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine U.S. Food and Drug Administration  Laurel Maryland USA"},{"name":"Retired"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"AVMA Guidelines for the Humane Slaughter of Animals.https:\/\/www.avma.org\/resources\u2010tools\/avma\u2010policies\/guidelines\u2010humane\u2010slaughter\u2010animals."},{"issue":"4","key":"e_1_2_10_3_1","first-page":"215","article-title":"Disease\u2010Induced Modifications of Drug Pharmacokinetics","volume":"3","author":"Barre J.","year":"1983","journal-title":"International Journal of Clinical Pharmacology Research"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s40262-023-01213-x","article-title":"Pharmacokinetic Alterations Associated With Critical Illness","volume":"62","author":"Castro D. 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