{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:52:54Z","timestamp":1789779174401,"version":"4.0.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2013,9,30]],"date-time":"2013-09-30T00:00:00Z","timestamp":1380499200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2013,9,30]],"date-time":"2013-09-30T00:00:00Z","timestamp":1380499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish Ministry of Education and Science","award":["CGL2011-25401"],"award-info":[{"award-number":["CGL2011-25401"]}]},{"name":"Lorch Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lett Appl Microbiol"],"published-print":{"date-parts":[[2014,1]]},"DOI":"10.1111\/lam.12157","type":"journal-article","created":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T07:02:39Z","timestamp":1378969359000},"page":"70-78","source":"Crossref","is-referenced-by-count":11,"title":["Quantification of relative proportions of intact cells in microbiological samples using the example of <i>Cryptosporidium parvum<\/i>\n oocysts"],"prefix":"10.1093","volume":"58","author":[{"given":"M.","family":"Agull\u00f3-Barcel\u00f3","sequence":"first","affiliation":[{"name":"Department of Microbiology; Faculty of Biology; Universitat de Barcelona; Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.A.","family":"Moss","sequence":"additional","affiliation":[{"name":"Center for Environmental Diagnostics and Bioremediation; University of West Florida; Pensacola FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Green","sequence":"additional","affiliation":[{"name":"Scottish Water; Juniper House; Heriot Watt Research Park; Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Scottish Water; Juniper House; Heriot Watt Research Park; Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Codony","sequence":"additional","affiliation":[{"name":"Laboratori de Microbiologia Sanit\u00e0ria i Mediambiental (MSM-Lab); Universitat Polit\u00e8cnica de Catalunya; Terrassa Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Lucena","sequence":"additional","affiliation":[{"name":"Department of Microbiology; Faculty of Biology; Universitat de Barcelona; Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Nocker","sequence":"additional","affiliation":[{"name":"Cranfield Water Science Institute; Cranfield University; Cranfield Bedfordshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,9,30]]},"reference":[{"key":"10.1111\/lam.12157-BIB0001|lam12157-cit-0001","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.2105\/AJPH.75.12.1433","article-title":"Moist heat inactivation of Cryptosporidium sp","volume":"75","author":"Anderson","year":"1985","journal-title":"Am J Public Health"},{"key":"10.1111\/lam.12157-BIB0002|lam12157-cit-0002","doi-asserted-by":"crossref","first-page":"5655","DOI":"10.1016\/j.watres.2012.07.057","article-title":"Comparison of UV\/H2O2 and UV\/TiO2 for the degradation of metaldehyde: kinetics and the impact of background organics","volume":"46","author":"Autin","year":"2012","journal-title":"Water Res"},{"key":"10.1111\/lam.12157-BIB0003|lam12157-cit-0003","first-page":"389","article-title":"Inactivation of Cryptosporidium parvum by medium pressure ultraviolet light in finished drinking water","volume":"A","author":"Bolton","year":"1998","journal-title":"J Am Water Works Assoc"},{"key":"10.1111\/lam.12157-BIB0004|lam12157-cit-0004","doi-asserted-by":"crossref","first-page":"6856","DOI":"10.1128\/AEM.00540-09","article-title":"Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable cryptosporidium oocysts","volume":"75","author":"Brescia","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0005|lam12157-cit-0005","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1002\/j.1551-8833.1999.tb08602.x","article-title":"Medium-pressure UV light for oocyst inactivation","volume":"91","author":"Bukhari","year":"1999","journal-title":"J Am Water Works Assoc"},{"key":"10.1111\/lam.12157-BIB0006|lam12157-cit-0006","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.mimet.2011.07.016","article-title":"Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment","volume":"87","author":"Contreras","year":"2011","journal-title":"J Microbiol Methods"},{"key":"10.1111\/lam.12157-BIB0007|lam12157-cit-0007","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1111\/j.1365-2672.2004.02281.x","article-title":"Survival of Cryptosporidium species in environments relevant to foods and beverages","volume":"96","author":"Dawson","year":"2004","journal-title":"J Appl Microbiol"},{"key":"10.1111\/lam.12157-BIB0008|lam12157-cit-0008","doi-asserted-by":"crossref","first-page":"2952","DOI":"10.1021\/ac001508n","article-title":"Detection of viable Cryptosporidium parvum using DNA-modified liposomes in a microfluidic chip","volume":"73","author":"Esch","year":"2001","journal-title":"Anal Chem"},{"key":"10.1111\/lam.12157-BIB0009|lam12157-cit-0009","doi-asserted-by":"crossref","first-page":"2732","DOI":"10.1128\/AEM.60.8.2732-2735.1994","article-title":"Effect of high temperature on infectivity of Cryptosporidium parvum oocysts in water","volume":"60","author":"Fayer","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0010|lam12157-cit-0010","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vetpar.2004.09.004","article-title":"Cryptosporidium: a water-borne zoonotic parasite","volume":"126","author":"Fayer","year":"2004","journal-title":"Vet Parasitol"},{"key":"10.1111\/lam.12157-BIB0011|lam12157-cit-0011","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.2307\/3284666","article-title":"Infectivity of Cryptosporidium parvum oocysts stored in water at environmental temperatures","volume":"84","author":"Fayer","year":"1998","journal-title":"J Parasitol"},{"key":"10.1111\/lam.12157-BIB0012|lam12157-cit-0012","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jviromet.2010.06.011","article-title":"Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR","volume":"168","author":"Fittipaldi","year":"2010","journal-title":"J Virol Methods"},{"key":"10.1111\/lam.12157-BIB0013|lam12157-cit-0013","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1550-7408.2011.00557.x","article-title":"Discrimination of Viable Acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction","volume":"58","author":"Fittipaldi","year":"2011","journal-title":"J Eukaryot Microbiol"},{"key":"10.1111\/lam.12157-BIB0014|lam12157-cit-0014","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.mimet.2012.08.007","article-title":"Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification","volume":"91","author":"Fittipaldi","year":"2012","journal-title":"J Microbiol Methods"},{"key":"10.1111\/lam.12157-BIB0015|lam12157-cit-0015","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s00248-005-0091-5","article-title":"Bacterial abundance, activity, and viability in the eutrophic river Warnow northeast Germany","volume":"51","author":"Freese","year":"2006","journal-title":"Microb Ecol"},{"key":"10.1111\/lam.12157-BIB0016|lam12157-cit-0016","doi-asserted-by":"crossref","first-page":"4475","DOI":"10.1128\/AEM.65.10.4475-4483.1999","article-title":"Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria","volume":"65","author":"Gasol","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0017|lam12157-cit-0017","doi-asserted-by":"crossref","first-page":"4325","DOI":"10.1016\/j.watres.2010.06.030","article-title":"Molecular detection of pathogens in water - The pros and cons of molecular techniques","volume":"44","author":"Girones","year":"2010","journal-title":"Water Res"},{"key":"10.1111\/lam.12157-BIB0018|lam12157-cit-0018","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.watres.2005.10.030","article-title":"Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: a review","volume":"40","author":"Hijnen","year":"2006","journal-title":"Water Res"},{"key":"10.1111\/lam.12157-BIB0019|lam12157-cit-0019","doi-asserted-by":"crossref","first-page":"3844","DOI":"10.1128\/AEM.63.10.3844-3850.1997","article-title":"Assessment of a dye permeability assay for determination of inactivation rates of Cryptosporidium parvum oocysts","volume":"63","author":"Jenkins","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0020|lam12157-cit-0020","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1017\/S0031182006001491","article-title":"Critical processes affecting Cryptosporidium oocyst survival in the environment","volume":"134","author":"King","year":"2007","journal-title":"Parasitol"},{"key":"10.1111\/lam.12157-BIB0021|lam12157-cit-0021","doi-asserted-by":"crossref","first-page":"3848","DOI":"10.1128\/AEM.71.7.3848-3857.2005","article-title":"Environmental temperature controls\u00a0Cryptosporidium\u00a0oocyst metabolic rate and associated retention of infectivity","volume":"71","author":"King","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0022|lam12157-cit-0022","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1021\/la047963m","article-title":"Role of surface proteins in the deposition kinetics of Cryptosporidium parvum oocysts","volume":"21","author":"Kuznar","year":"2005","journal-title":"Langmuir"},{"key":"10.1111\/lam.12157-BIB0023|lam12157-cit-0023","doi-asserted-by":"crossref","first-page":"5941","DOI":"10.1016\/j.watres.2012.08.014","article-title":"Comparison of propidium monoazide-quantitative PCR and reverse transcription quantitative PCR for viability detection of fresh Cryptosporidium oocysts following disinfection and after long-term storage in water samples","volume":"46","author":"Liang","year":"2012","journal-title":"Water Res"},{"key":"10.1111\/lam.12157-BIB0024|lam12157-cit-0024","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1128\/AEM.68.7.3509-3513.2002","article-title":"Large fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols for determination and ecological significance","volume":"68","author":"Luna","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0025|lam12157-cit-0025","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s00253-009-2373-1","article-title":"Method to detect only viable cells in microbial ecology","volume":"86","author":"Luo","year":"2010","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1111\/lam.12157-BIB0026|lam12157-cit-0026","volume-title":"Risk Assessment of Cryptosporidium in Drinking Water","author":"Medema","year":"2009"},{"key":"10.1111\/lam.12157-BIB0027|lam12157-cit-0027","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.mimet.2007.04.014","article-title":"Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR","volume":"70","author":"Nocker","year":"2007","journal-title":"J Microbiol Methods"},{"key":"10.1111\/lam.12157-BIB0028|lam12157-cit-0028","doi-asserted-by":"crossref","first-page":"4318","DOI":"10.1128\/AEM.02800-09","article-title":"Use of propidium monoazide in reverse transcriptase pcr to distinguish between infectious and noninfectious enteric viruses in water samples","volume":"76","author":"Parshionikar","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0029|lam12157-cit-0029","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1111\/j.1550-7408.2004.tb00291.x","article-title":"Irreversible UV inactivation of Cryptosporidium spp. despite the presence of UV repair genes1","volume":"51","author":"Rochelle","year":"2004","journal-title":"J Eukaryot Microbiol"},{"key":"10.1111\/lam.12157-BIB0030|lam12157-cit-0030","doi-asserted-by":"crossref","first-page":"1854","DOI":"10.1111\/jam.12169","article-title":"Long-range quantitative PCR for determining inactivation of adenovirus 2 by ultraviolet light","volume":"114","author":"Rodriguez","year":"2013","journal-title":"J Appl Microbiol"},{"key":"10.1111\/lam.12157-BIB0031|lam12157-cit-0031","doi-asserted-by":"crossref","first-page":"3376","DOI":"10.3390\/ijerph7093376","article-title":"Different length (DL) qPCR for quantification of cell killing by UV-induced DNA damage","volume":"7","author":"Rudi","year":"2010","journal-title":"Int J Environ Res Public Health"},{"key":"10.1111\/lam.12157-BIB0032|lam12157-cit-0032","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1111\/j.1365-2672.2012.05384.x","article-title":"PCR inhibitors - occurrence, properties and removal","volume":"113","author":"Schrader","year":"2012","journal-title":"J Appl Microbiol"},{"key":"10.1111\/lam.12157-BIB0033|lam12157-cit-0033","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ab.2011.06.027","article-title":"Polymerase chain reaction amplification length-dependent ethidium monoazide suppression power for heat-killed cells of Enterobacteriaceae","volume":"418","author":"Soejima","year":"2011","journal-title":"Anal Biochem"},{"key":"10.1111\/lam.12157-BIB0034|lam12157-cit-0034","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1128\/AEM.62.9.3385-3390.1996","article-title":"Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR","volume":"62","author":"Stinear","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/lam.12157-BIB0035|lam12157-cit-0035","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1099\/jmm.0.46678-0","article-title":"Real-time PCR detection and speciation of Cryptosporidium infection using Scorpion probes","volume":"55","author":"Stroup","year":"2006","journal-title":"J Med Microbiol"},{"key":"10.1111\/lam.12157-BIB0036|lam12157-cit-0036","volume-title":"Ultraviolet Disinfection Guidance Manual for the Final Long Term 2 Enhanced Surface Water Treatment Rule","author":"U.S. Environmental Protection Agency","year":"2006"},{"key":"10.1111\/lam.12157-BIB0037|lam12157-cit-0037","doi-asserted-by":"crossref","first-page":"5544","DOI":"10.1128\/AEM.66.12.5544-5548.2000","article-title":"Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments","volume":"66","author":"Yokomaku","year":"2000","journal-title":"Appl Environ Microbiol"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Flam.12157","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Flam.12157","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.1111\/lam.12157\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T15:01:12Z","timestamp":1676386872000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/58\/1\/70\/6699573"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,30]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"URL":"https:\/\/doi.org\/10.1111\/lam.12157","archive":["Portico"],"relation":{},"ISSN":["0266-8254"],"issn-type":[{"value":"0266-8254","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9,30]]}}}