{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:01:48Z","timestamp":1790686908570,"version":"4.1.0"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study aimed to investigate the molecular mechanisms of carbapenem and colistin resistance in K. pneumoniae and E. coli isolates obtained from hospitalized patients in Carthagene International Hospital of Tunis. A total of 25 K. pneumoniae and 2 E. coli clinical isolates with reduced susceptibility to carbapenems were recovered. Susceptibility testing and phenotypic screening tests were carried out. ESBL, AmpC, carbapenemase and other antibiotic resistance genes were sought by PCR-sequencing. The presence of plasmid-mediated colistin resistance genes (mcr-1-8) was examined by PCR and the nucleotide sequence of the mgrB gene was determined. The analysis of plasmid content was performed by PCR-Based Replicon Typing (PBRT). The clonality of isolates was assessed by PFGE and multilocus sequence typing (MLST). All of the isolates produced carbapenemase activity. They showed a great variation in the distribution of ESBL, AmpC, carbapenemase and other plasmid-mediated resistance determinants. K. pneumoniae isolates carried blaNDM-1 (n = 11), blaOXA-48 (n = 11), blaNDM-1 + blaOXA-48 (n = 1), blaNDM-1 + blaVIM-1 (n = 1), blaOXA-204 (n = 1), along with blaCTX-M, blaOXA, blaTEM, blaCMY, blaDHA and blaSHV genes variants on conjugative plasmid of IncL\/M, IncR, IncFIIK, IncFIB, and IncHI1 types. Three sequence types ST101, ST307 and ST15 were identified. The mgrB alteration g109a (G37S) was detected in a single colistin-resistant, NDM-1 and OXA-48-coproducing K. pneumoniae isolate. The two E. coli isolates belonged to ST95, co-produced NDM-1 and CTX-M-15, and harboured plasmid of IncFII and IncFIB types. To our knowledge, this is the first report in Tunisia of NDM-1, OXA-48, and CTX-M-15 coexistence in colistin-resistant K. pneumoniae ST15.<\/jats:p>","DOI":"10.1111\/lam.13658","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T15:08:28Z","timestamp":1643123308000},"page":"729-740","source":"Crossref","is-referenced-by-count":18,"title":["Co-occurrence of genes encoding carbapenemase, ESBL, pAmpC and non-\u03b2-Lactam resistance among Klebsiella pneumonia and E. coli clinical isolates in Tunisia"],"prefix":"10.1093","volume":"74","author":[{"given":"R.","family":"Ben Sallem","sequence":"first","affiliation":[{"name":"Laboratory of Microorganisms and Active Biomolecules Faculty of Sciences of Tunis University of Tunis El Manar Tunis 2092 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Laribi","sequence":"additional","affiliation":[{"name":"Laboratory of Microorganisms and Active Biomolecules Faculty of Sciences of Tunis University of Tunis El Manar Tunis 2092 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Arfaoui","sequence":"additional","affiliation":[{"name":"Laboratory of Microorganisms and Active Biomolecules Faculty of Sciences of Tunis University of Tunis El Manar Tunis 2092 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ben Khelifa Melki","sequence":"additional","affiliation":[{"name":"Service of Biology Carthagene International Hospital of Tunisia Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. I.","family":"Ouzari","sequence":"additional","affiliation":[{"name":"Laboratory of Microorganisms and Active Biomolecules Faculty of Sciences of Tunis University of Tunis El Manar Tunis 2092 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ben Slama","sequence":"additional","affiliation":[{"name":"Laboratory of Microorganisms and Active Biomolecules Faculty of Sciences of Tunis University of Tunis El Manar Tunis 2092 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Naas","sequence":"additional","affiliation":[{"name":"Bacteriology-Hygiene Unit Bic\u00eatre Hospital Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae Assistance Publique - H\u00f4pitaux de Paris Le Kremlin-Bic\u00eatre France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7405-0682","authenticated-orcid":false,"given":"N.","family":"Klibi","sequence":"additional","affiliation":[{"name":"Laboratory of Microorganisms and Active Biomolecules Faculty of Sciences of Tunis University of Tunis El Manar Tunis 2092 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,5,1]]},"reference":[{"key":"2023011712225103900_lam13658-bib-0001","doi-asserted-by":"crossref","first-page":"2904","DOI":"10.1093\/jac\/dky272","article-title":"mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015","volume":"73","author":"AbuOun","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"2023011712225103900_lam13658-bib-0002","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jgar.2019.06.001","article-title":"Successful 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