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Herein, the antimicrobial potential of Thymbra capitata essential oil (EO) against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the T. capitata EO was first investigated on a group of clinical isolates from septicaemias, diabetic foot ulcers and osteomyelitis. Biofilms were incubated with the EO at the MLC and its anti-biofilm potential was investigated. A strong antimicrobial activity was observed, with MIC and MLC values between 0\u00b732 and 0\u00b764 mg l\u22121. However, the concentration of EO necessary for the eradication of planktonic cells was insufficient to significantly reduce the biofilm biomass of some isolates. Nevertheless, cell culturability and overall cellular metabolism was strongly reduced in all biofilms tested, only when the EO was tested. Contrary to the tested antibiotics, T. capitata EO showed a significant antimicrobial activity against MRSA biofilms, by reducing cellular metabolism and cellular culturability.<\/jats:p>","DOI":"10.1111\/lam.13665","type":"journal-article","created":{"date-parts":[[2022,2,6]],"date-time":"2022-02-06T18:33:22Z","timestamp":1644172402000},"page":"787-795","source":"Crossref","is-referenced-by-count":5,"title":["Thymbra capitata essential oil has a significant antimicrobial activity against methicillin-resistant Staphylococcus aureus pre-formed biofilms"],"prefix":"10.1093","volume":"74","author":[{"given":"L.","family":"Almeida","sequence":"first","affiliation":[{"name":"Centre of Biological Engineering (CEB) Laboratory of Research in Biofilms Ros\u00e1rio Oliveira (LIBRO) University of Minho Braga Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Lopes","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB) 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