{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:55:49Z","timestamp":1768438549247,"version":"3.49.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Curtobacterium genus is a member of the family Microbacteriaceae, and Curtobacterium species are recognized as plant pathogens. The aim of this study was to investigate a dubious result of species identification for an infection located on a catheter tip of a patient with Covid-19. A strain isolated from a catheter tip sample, identified by VITEK\u00ae 2 as Cronobacter spp., was submitted to polyphasic analysis: Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) using VITEK\u00ae MS, real-time polymerase chain reaction targeting dnaG gene, and 16S rRNA full gene Sanger sequencing analysis for confirmation. The strain presented negative result using qPCR and could not identified by MALDI-TOF MS. 16S rRNA full gene Sanger sequencing analysis identified the strain as Curtobacterium spp. The Gram-variable characteristic (Gram-negative instead of Gram-positive) of the isolated strain was the responsible for the misidentification by VITEK\u00ae 2 and VITEK\u00ae MS did not identify the strain. 16S rRNA full gene sequencing analysis identified the strain as Curtobacterium genus, but other complementary techniques are necessary to identify at species level.<\/jats:p>","DOI":"10.1111\/lam.13741","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T05:04:27Z","timestamp":1653282267000},"page":"396-400","source":"Crossref","is-referenced-by-count":7,"title":["Case report of Curtobacterium isolated from a catheter tip sample misidentified as Cronobacter"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2974-7311","authenticated-orcid":false,"given":"L.M.P.S.","family":"Braga","sequence":"first","affiliation":[{"name":"Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil"},{"name":"Federal University of Rio de Janeiro Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7479-2838","authenticated-orcid":false,"given":"B.A.A.","family":"Saad","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso do Sul Campo Grande MS Brazil"},{"name":"Regional Hospital of Mato Grosso do Sul Campo Grande MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1430-2881","authenticated-orcid":false,"given":"C.T.F.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Regional Hospital of Mato Grosso do Sul Campo Grande MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3004-2039","authenticated-orcid":false,"given":"C.E.","family":"Volpe-Chaves","sequence":"additional","affiliation":[{"name":"Regional Hospital of Mato Grosso do Sul Campo Grande MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5061-6589","authenticated-orcid":false,"given":"M.L.G.G.","family":"Lacerda","sequence":"additional","affiliation":[{"name":"Regional Hospital of Mato Grosso do Sul Campo Grande MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6709-0712","authenticated-orcid":false,"given":"S.J.","family":"Forsythe","sequence":"additional","affiliation":[{"name":"foodmicrobe.com Adams Hill Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0035-2439","authenticated-orcid":false,"given":"J.","family":"Venturini","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso do Sul Campo Grande MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8960-6716","authenticated-orcid":false,"given":"S.M.V.L.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso do Sul Campo Grande MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8925-7712","authenticated-orcid":false,"given":"A.M.M.","family":"Paniago","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso do Sul Campo Grande MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1873-4056","authenticated-orcid":false,"given":"L.V.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7131-6155","authenticated-orcid":false,"given":"R.V.S.L.","family":"de Miranda","sequence":"additional","affiliation":[{"name":"Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil"},{"name":"National Institute of Quality Control in Health, Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0331-5126","authenticated-orcid":false,"given":"C.M.F.","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1121-7312","authenticated-orcid":false,"given":"M.L.L.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil"},{"name":"National Institute of Quality Control in Health, Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,8,1]]},"reference":[{"key":"2023011712241410100_lam13741-bib-0001","volume-title":"EN ISO 22964:2017 Microbiology of the Food Chain - Horizontal Method for the Detection of Cronobacter spp","author":"Anonymous","year":"2017"},{"key":"2023011712241410100_lam13741-bib-0002","volume-title":"Molecular Typing in Bacterial Infections","author":"Brand\u00e3o","year":"2022"},{"key":"2023011712241410100_lam13741-bib-0003","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.3201\/eid2410.180373","article-title":"Fatal Cronobacter sakazakii sequence type 494 meningitis in a newborn","volume":"24","author":"Chaves","year":"2018","journal-title":"Brazil Emerg Infect Dis"},{"key":"2023011712241410100_lam13741-bib-0004","volume-title":"Bacteriological Analytical Manual","author":"Chen","year":"2012"},{"key":"2023011712241410100_lam13741-bib-0005","first-page":"3545","article-title":"Reclassification of Corynebacterium flaccumfaciens, Corynebacterium betae, Corynebacterium oortii and Corynebacterium poinsettiae in the genus Curtobacterium, as Curtobacterium flaccumfaciens comb. nov","volume":"129","author":"Collins","year":"1983","journal-title":"J Gen Microbiol"},{"key":"2023011712241410100_lam13741-bib-0006","doi-asserted-by":"crossref","DOI":"10.1016\/j.mimet.2022.106419","article-title":"Assessment of VITEK\u00ae 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility","volume":"194","author":"Costa","year":"2022","journal-title":"J Microbiol Methods"},{"key":"2023011712241410100_lam13741-bib-0007","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1128\/JCM.00340-11","article-title":"Curtobacterium flaccumfaciens septic arthritis following puncture with a Coxspur hawthorn thorn","volume":"49","author":"Francis","year":"2011","journal-title":"J Clin Microbiol"},{"key":"2023011712241410100_lam13741-bib-0008","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1128\/JCM.43.3.1032-1036.2005","article-title":"First description of Curtobacterium spp. isolated from human clinical specimens","volume":"43","author":"Funke","year":"2005","journal-title":"J Clin Microbiol"},{"key":"2023011712241410100_lam13741-bib-0009","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1089\/fpd.2010.0719","article-title":"Molecular epidemiological survey of Citrobacter freundii misidentified as Cronobacter spp. (Enterobacter sakazakii) and Enterobacter hormaechei isolated from powdered infant milk formula","volume":"8","author":"Giammanco","year":"2011","journal-title":"Foodborne Pathog Dis"},{"key":"2023011712241410100_lam13741-bib-0010","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s13256-021-03241-2","article-title":"Bacteremia caused by Enterobacter asburiae misidentified biochemically as Cronobacter sakazakii and accurately identified by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry: a case report","volume":"16","author":"Horinouchi","year":"2022","journal-title":"J Med Case Reports"},{"key":"2023011712241410100_lam13741-bib-0011","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/s12866-016-0768-6","article-title":"Comparative study of Cronobacter identification according to phenotyping methods","volume":"16","author":"Jackson","year":"2016","journal-title":"BMC Microbiol"},{"key":"2023011712241410100_lam13741-bib-0012","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF01731581","article-title":"A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences","volume":"16","author":"Kimura","year":"1980","journal-title":"J Mol Evol"},{"key":"2023011712241410100_lam13741-bib-0013","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol Biol Evol"},{"key":"2023011712241410100_lam13741-bib-0014","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/BF00873089","article-title":"Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA","volume":"64","author":"Park","year":"1993","journal-title":"Antonie Van Leeuwenhoek"},{"issue":"Pt 12","key":"2023011712241410100_lam13741-bib-0015","doi-asserted-by":"crossref","first-page":"3659","DOI":"10.1099\/mic.0.2008\/021980-0","article-title":"Characterization of an extended-spectrum beta-lactamase Enterobacter hormaechei nosocomial outbreak, and other Enterobacter hormaechei misidentified as Cronobacter (Enterobacter) sakazakii","volume":"154","author":"Townsend","year":"2008","journal-title":"Microbiology (Reading)"},{"key":"2023011712241410100_lam13741-bib-0016","doi-asserted-by":"crossref","first-page":"21","DOI":"10.53393\/rial.2012.71.32386","article-title":"Phenotypic profiles and detection of target genes by PCR in isolates from different sources and reference strains, identified as Cronobacter spp. (Enterobacter sakazakii)","volume":"71","author":"Warnken","year":"2012","journal-title":"Rev Inst Adolfo Lutz"},{"key":"2023011712241410100_lam13741-bib-0017","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1099\/ijsem.0.001755","article-title":"Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies","volume":"67","author":"Yoon","year":"2017","journal-title":"Int J Syst Evol Microbiol"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/lam.13741","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/lam.13741","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/75\/2\/396\/48738487\/lambio0396.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/75\/2\/396\/48738487\/lambio0396.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T18:49:59Z","timestamp":1727290199000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/75\/2\/396\/6989434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,1]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,8,1]]}},"URL":"https:\/\/doi.org\/10.1111\/lam.13741","relation":{},"ISSN":["1472-765X","0266-8254"],"issn-type":[{"value":"1472-765X","type":"electronic"},{"value":"0266-8254","type":"print"}],"subject":[],"published-other":{"date-parts":[[2022,8,1]]},"published":{"date-parts":[[2022,8,1]]}}}