{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:55:14Z","timestamp":1773273314888,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100007063","name":"New Delhi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007063","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["5\/8\/5\/38\/2014-ECD-I"],"award-info":[{"award-number":["5\/8\/5\/38\/2014-ECD-I"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A multiplex-nested PCR (M-nested PCR) targeting mpt64 (Rv1980c) + IS6110 was designed to detect Mycobacterium tuberculosis (Mtb) DNA within urine (n = 35), endometrial biopsies (n = 22) and menstrual blood (n = 3) of male\/female UGTB patients, and results were compared with M-PCR using the same targets. Detection limit of the purified Mtb DNA was found to be 1 fg by M-nested PCR, which was 106-fold lower than M-PCR. Moreover, sensitivities of 100% and 81\u00b78% were obtained in confirmed (n = 5) and clinically suspected UGTB (n = 55) cases, respectively, by M-nested PCR, with a specificity of 97\u00b71% (n = 70). Sensitivities attained by M-nested PCR were significantly higher (p &amp;lt; 0\u00b705) than M-PCR in both clinically suspected and total UGTB (n = 60) cases. To confirm the true PCR-negative results, an internal amplification control, that is, human \u03b2-globin gene (hbb) was incorporated in the M-nested PCR\/M-PCR assays, wherein all the clinical specimens (positive\/negative for mpt64\/IS6110) were found to be positive for hbb. Some UGTB specimens (n = 35) were also subjected to GeneXpert\u00ae MTB\/RIF assay that revealed a significantly lower (p &amp;lt; 0\u00b7001) sensitivity (17\u00b71 vs 88\u00b76%) than M-nested PCR, although high specificity (100%) was attained with GeneXpert. After validating the results in a higher number of UGTB specimens, our M-nested PCR may be translated into an attractive diagnostic kit.<\/jats:p>","DOI":"10.1111\/lam.13758","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T08:19:28Z","timestamp":1654676368000},"page":"857-868","source":"Crossref","is-referenced-by-count":6,"title":["Diagnosis of urogenital tuberculosis by multiplex-nested PCR targeting mpt64 (Rv1980c) and IS6110: comparison with multiplex PCR and GeneXpert\u00ae MTB\/RIF"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1364-1080","authenticated-orcid":false,"given":"E.","family":"Kamra","sequence":"first","affiliation":[{"name":"Centre for Biotechnology Maharshi Dayanand University Rohtak India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Alam","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Maharshi Dayanand University Rohtak 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