{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:14:54Z","timestamp":1787015694690,"version":"build-2736575974"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["SA51-006646"],"award-info":[{"award-number":["SA51-006646"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Gonorrhoea infections are frequently diagnosed at extragenital locations in asymptomatic individuals and are historically related to poor recovery in culture, which hinders antimicrobial susceptibility testing. The aim of this study was to evaluate recovery rates of Neisseria gonorrhoeae by culture among asymptomatic individuals who tested positive by nucleic acid amplification tests between 2018 and 2019 in Barcelona (Spain). In total, 10 396 individuals were tested for N. gonorrhoeae on first-void urine, rectal, pharyngeal and\/or vaginal swabs depending on sexual behaviour. Overall infection prevalence was 5\u00b75% (95% confidence interval [CI] 5\u00b70\u20135\u00b79). Seven hundred and ten samples were positive corresponding to 567 individuals. The most common site of infection was the pharynx (71\u00b73%), followed by rectum (23\u00b71%) and genitals (4\u00b77%) (P &amp;lt; 0\u00b70001). The N. gonorrhoeae recovery rate in culture, time from positive screening to culture specimen and inoculation delay were calculated. Recovery rate was 21\u00b77% in pharynx, 66\u00b79% in rectum and 37\u00b70% in genitals (25\u00b70% vagina, 71\u00b74% urethra) (P &amp;lt; 0\u00b70001). Median culture collection time was 1 [0; 3] days, and median inoculation delay was 5\u00b701 [4\u00b799\u20137\u00b799] h, with no impact on N. gonorrhoeae recovery, P = 0\u00b78367 and P = 0\u00b77670, respectively. Despite efforts towards optimizing pre-analytical conditions, the N. gonorrhoeae recovery rate in asymptomatic individuals is unacceptably low (especially for pharynx), representing a problem for monitoring antimicrobial-resistant infections.<\/jats:p>","DOI":"10.1111\/lam.13789","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T04:24:38Z","timestamp":1658377478000},"page":"1215-1224","source":"Crossref","is-referenced-by-count":9,"title":["Neisseria gonorrhoeae culture growth rates from asymptomatic individuals with a positive nucleic acid amplification test"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2656-2656","authenticated-orcid":false,"given":"P.","family":"Nadal-Baron","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology Vall d'Hebron University Hospital Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Salmer\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Vall d'Hebron University Hospital Barcelona Spain"},{"name":"Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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N.","family":"Larrosa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Vall d'Hebron University Hospital Barcelona Spain"},{"name":"Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Pumarola","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Vall d'Hebron University Hospital Barcelona Spain"},{"name":"Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6354-0149","authenticated-orcid":false,"given":"Y.","family":"Hoyos-Mallecot","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Vall d'Hebron University Hospital Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"on behalf of the Drassanes Expr\u00e9s Collaborative 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