{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T15:11:32Z","timestamp":1782141092403,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T00:00:00Z","timestamp":1350864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Faculty of Medicine"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chemoresistance and advanced tumour stage at time of diagnosis are the major reasons for poor treatment results in hepatoblastoma (<jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>) and paediatric hepatocellular carcinoma (<jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>). Positive results with transplantation of liver and bone marrow revealed the impact of the immune system on the treatment of liver malignancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>Cytotoxic\u2010immune\u2010cells\u2010like natural killer (<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>) and T cells are major player in the defence against developing tumours. This study aimed to specifically analyse the ability of ex\u2010vivo expanded \u03b3\u03b4 T cells to recognise and lyse <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> cell lines in coculture assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cell viability after treatment with \u03b3\u03b4 T cells was evaluated with two <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">HUH<\/jats:styled-content>6 and HepT1) and one <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> cell line (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">AFW<\/jats:styled-content>1) using a <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>\u2010based cytotoxicity assay. The binding of T cells to target cells was monitored using immunofluorescence microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Incubation of hepatic tumour cell lines with \u03b3\u03b4 T cells led to a significant decrease in tumour cell viability. This was enhanced by zoledronic acid and histone deacetylase inhibitors. <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>110, an Ep<jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3\u2010bispecific Bi<jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content> antibody could bluntly enhance tumour cell lysis close to completion. \u03b3\u03b4 T cells efficiently interacted with <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> cells in a spheroid culture model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Bispecific antibodies such as <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>110 might be used to intensify the antitumoural effect of \u03b3\u03b4 T cells in context of adoptive immune cell transfer. Optimised immunotherapeutic strategies might therefore improve the outcome of high risk hepatoblastoma and hepatocellular carcinoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12011","type":"journal-article","created":{"date-parts":[[2012,10,23]],"date-time":"2012-10-23T02:05:55Z","timestamp":1350957955000},"page":"127-136","source":"Crossref","is-referenced-by-count":40,"title":["The activity of \u03b3\u03b4 T cells against paediatric liver tumour cells and spheroids in cell culture"],"prefix":"10.1111","volume":"33","author":[{"given":"Alexander","family":"Hoh","sequence":"first","affiliation":[{"name":"Department of Paediatric Surgery and Urology University Childre\u01f9s Hospital Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Dewerth","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Urology University Childre\u01f9s Hospital Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Vogt","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Urology University Childre\u01f9s Hospital Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Wenz","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Urology University Childre\u01f9s Hospital Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick A.","family":"Baeuerle","sequence":"additional","affiliation":[{"name":"Amgen Research Munich Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven W.","family":"Warmann","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Urology University Childre\u01f9s Hospital Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joerg","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Urology University Childre\u01f9s Hospital Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorin","family":"Armeanu\u2010Ebinger","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Urology University Childre\u01f9s Hospital Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.06.102"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1967)20:3<396::AID-CNCR2820200308>3.0.CO;2-O"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.06.155"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(83)80005-7"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00247-005-0067-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(98)90005-7"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2008-0011"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0708857"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2011.10.011"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.3.1338"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240802419328"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.975"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/587315"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)80032-X"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.4.2144"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/375155a0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/323600"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1655"},{"key":"e_1_2_6_20_1","first-page":"1","article-title":"Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma","volume":"67","author":"Doi I","year":"1976","journal-title":"Gann"},{"key":"e_1_2_6_21_1","first-page":"809","article-title":"Characterization of the continuous cell line HepT1 derived from a human hepatoblastoma","volume":"74","author":"Pietsch T","year":"1996","journal-title":"Lab Invest"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038223"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2010.03.023"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-011-2988-z"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013474"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000318055"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdp314"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/732893."},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2008.11.002"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-4252"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.2741\/e346"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2007.00777.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.22.4857"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.20404"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-12-3665"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240701644000"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4912"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.3.1.14193"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2011.04.002"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-6013"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2005.07.010"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23365"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/38664"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/35106079"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182372299"},{"key":"e_1_2_6_46_1","first-page":"575","article-title":"Complete remission of lung metastasis following adoptive immunotherapy using activated autologous gammadelta T\u2010cells in a patient with renal cell carcinoma","volume":"30","author":"Kobayashi H","year":"2010","journal-title":"Anticancer Res"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-011-1021-7"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.293"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00595-010-4478-7"}],"container-title":["Liver International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fliv.12011","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fliv.12011","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/liv.12011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T04:53:12Z","timestamp":1700628792000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/liv.12011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,22]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1111\/liv.12011"],"URL":"https:\/\/doi.org\/10.1111\/liv.12011","archive":["Portico"],"relation":{},"ISSN":["1478-3223","1478-3231"],"issn-type":[{"value":"1478-3223","type":"print"},{"value":"1478-3231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,22]]}}}