{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:17:38Z","timestamp":1772983058120,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,12,26]],"date-time":"2012-12-26T00:00:00Z","timestamp":1356480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Danish Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background\/Aim<\/jats:title><jats:p>We investigated nine novel biomarkers of extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>) remodelling in a rat model of liver fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liver fibrosis was induced in 52 male Wistar rats by inhalation of carbon tetrachloride and the level of hepatic fibrosis was assessed by Sirius red staining compared with controls. The novel serum biochemical markers assessed in the model were type I\u2010(C1M), type III\u2010(C3M), type IV\u2010(C4M) and type VI\u2010(C6M) collagen, citrullinated vimentin (<jats:styled-content style=\"fixed-case\">VICM<\/jats:styled-content>) and biglycan (<jats:styled-content style=\"fixed-case\">BGM<\/jats:styled-content>) all protein fragments generated by matrix metalloproteinases; and formation markers of type III\u2010(P3NP), type VI (P4NP 7S) and type V (P5CP) collagen; hepatic m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> type I collagen alpha\u20101 chain levels, serum potassium, sodium, osmolarity, alanine aminotransferase, lactate dehydrogenase, albumin and creatinine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Stratification of the <jats:styled-content style=\"fixed-case\">CCl<\/jats:styled-content><jats:sub>4<\/jats:sub>\u2010treated rats according to total hepatic collagen showed that the degradation markers were significantly elevated in mild to severe fibrosis except for C6M which was also elevated in early fibrosis (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). The highest Z\u2010scores in early and moderate fibrosis were provided by P4NP 7S and alanine aminotransferase. All nine markers of <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> remodelling were highly related to the extent of liver fibrosis induced by <jats:styled-content style=\"fixed-case\">CCl<\/jats:styled-content><jats:sub>4<\/jats:sub>. The novel collagen formation marker, P4NP 7S, was reliable for the detection of early fibrosis, while the combination of the two markers, C6M and P5CP provided the best correlation with hepatic fibrosis in all fibrosis levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>As the markers can be used for translational science, these markers may provide valuable information for the evaluation of liver fibrosis in clinical settings.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12044","type":"journal-article","created":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T19:43:49Z","timestamp":1354650229000},"page":"439-447","source":"Crossref","is-referenced-by-count":49,"title":["Protein fingerprinting of the extracellular matrix remodelling in a rat model of liver fibrosis\u2014a serological evaluation"],"prefix":"10.1111","volume":"33","author":[{"given":"Diana J.","family":"Leeming","sequence":"first","affiliation":[{"name":"Nordic Bioscience Fibrosis Biology and Biomarkers Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inger","family":"Byrjalsen","sequence":"additional","affiliation":[{"name":"Nordic Bioscience Fibrosis Biology and Biomarkers Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wladimiro","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Service of Biochemistry and Molecular Genetics Hospital Clinic, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS) Department of Physiological Sciences I University of Barcelona Barcelona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd) Department of Physiological Sciences I University of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Nordic Bioscience Fibrosis Biology and Biomarkers Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten A.","family":"Karsdal","sequence":"additional","affiliation":[{"name":"Nordic Bioscience Fibrosis Biology and Biomarkers Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24282"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60383-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2008.01761.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2007.00092.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2008.07.010"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2009.01263.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-6-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-11-91"},{"key":"e_1_2_7_10_1","first-page":"103","article-title":"Noninvasive assessment of liver fibrosis with the aspartate transaminase to platelet ratio index (APRI): Usefulness in patients with chronic liver disease: APRI in chronic liver disease","volume":"11","author":"Yilmaz Y","year":"2011","journal-title":"Hepat Mon"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2012.03.016"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2006.tb00292.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23654"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00429-4"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.111.188078"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2008.09.033"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90564.2008"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/13547500902777608"},{"key":"e_1_2_7_19_1","first-page":"793","article-title":"Novel combinations of Post\u2010Translational Modification (PTM) neo\u2010epitopes provide tissue\u2010specific biochemical markers \u2010 are they the cause or the consequence of the disease?","volume":"43","author":"Karsdal MA","year":"2010","journal-title":"Clin Chem"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-1536-4-19"},{"key":"e_1_2_7_21_1","first-page":"379","volume-title":"The Liver and the Kidney","author":"Clari\u00e0 J","year":"1999"},{"key":"e_1_2_7_22_1","volume-title":"Experimental Models of Cirrhosis and Ascites. 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