{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:58:13Z","timestamp":1787065093610,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T00:00:00Z","timestamp":1366848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2013,7]]},"abstract":"Abstract<\/jats:title>\n \n Background & Aims<\/jats:title>\n \n Acute liver failure (\n ALF<\/jats:styled-content>\n ) is a rare clinical syndrome with high mortality resulting from hepatocellular necrosis and loss of function. In seronegative hepatitis (\n SNH<\/jats:styled-content>\n ), a T\u2010cell\u2010rich infiltrate leads to immune\u2010mediated hepatocyte destruction, whereas in paracetamol poisoning, toxic metabolites induce hepatocyte necrosis, followed by a macrophage\u2010rich, lymphocytic infiltrate that is an important factor in driving repair and regeneration. The nature of the hepatic inflammatory infiltrate, key to\n ALF<\/jats:styled-content>\n pathogenesis and outcome, is determined by the recruitment of effector cells from blood, but the molecular basis of recruitment is poorly understood. To determine the phenotype of circulating and hepatic lymphocytes in patients with\n ALF<\/jats:styled-content>\n secondary to paracetamol overdose (\n POD<\/jats:styled-content>\n ) or\n SNH<\/jats:styled-content>\n and investigate the molecular basis of lymphocyte recruitment.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We used\n FACS<\/jats:styled-content>\n , immunohistochemistry and flow\u2010based adhesion assays to determine the regulation of lymphocyte adhesion.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n SNH<\/jats:styled-content>\n and\n POD<\/jats:styled-content>\n intrahepatic lymphocytes were \u03b1L\u03b22\n hi<\/jats:sup>\n ,\n CD<\/jats:styled-content>\n 69\n hi<\/jats:sup>\n and\n CD<\/jats:styled-content>\n 38\n hi<\/jats:sup>\n with a distinct homing phenotype being L\u2010selectin\n lo<\/jats:sup>\n ,\n CXCR<\/jats:styled-content>\n 3\n hi<\/jats:sup>\n and\n CCR<\/jats:styled-content>\n 5\n +<\/jats:sup>\n . Expression of chemokine ligands for the receptors\n CCR<\/jats:styled-content>\n 5,\n CXCR<\/jats:styled-content>\n 3 and\n CXCR<\/jats:styled-content>\n 6 and the adhesion molecules\n ICAM<\/jats:styled-content>\n \u20101,\n VCAM<\/jats:styled-content>\n \u20101 and\n VAP<\/jats:styled-content>\n \u20101 was markedly increased in the liver in\n ALF<\/jats:styled-content>\n . Lymphocytes isolated from the livers of patients with\n SNH<\/jats:styled-content>\n showed enhanced chemokine\u2010dependent adhesion and transmigration across the human hepatic endothelium\n in vitro<\/jats:italic>\n under flow and used a combination of \u03b21 and \u03b22 integrins to adhere to endothelium and \u03b22 integrins,\n CD<\/jats:styled-content>\n 31 and\n VAP<\/jats:styled-content>\n \u20101 to transmigrate.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Aetiology\u2010dependent combinations of adhesion molecules and chemokines expressed within tissue during\n ALF<\/jats:styled-content>\n recruit lymphocytes with a distinct homing phenotype.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/liv.12182","type":"journal-article","created":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T04:12:45Z","timestamp":1364789565000},"page":"852-863","source":"Crossref","is-referenced-by-count":20,"title":["The regulation of T\u2010cell recruitment to the human liver during acute liver failure"],"prefix":"10.1111","volume":"33","author":[{"given":"Ceren","family":"Tuncer","sequence":"first","affiliation":[{"name":"NIHR Biomedical Research Unit and Centre for Liver Research University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye H.","family":"Oo","sequence":"additional","affiliation":[{"name":"NIHR Biomedical Research Unit and Centre for Liver Research University of Birmingham Birmingham UK"},{"name":"The Liver Unit Queen Elizabeth Hospital Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"Murphy","sequence":"additional","affiliation":[{"name":"The Liver Unit Queen Elizabeth Hospital Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Adams","sequence":"additional","affiliation":[{"name":"NIHR Biomedical Research Unit and Centre for Liver Research University of Birmingham Birmingham UK"},{"name":"The Liver Unit Queen Elizabeth Hospital Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia F.","family":"Lalor","sequence":"additional","affiliation":[{"name":"NIHR Biomedical Research Unit and Centre for Liver Research University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60274-7"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22177"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2004.0982.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803417"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2010.02262.x"},{"key":"e_1_2_5_7_1","first-page":"305","article-title":"Acetaminophen\u2010induced hepatotoxicity in mice is dependent on Tlr9 and the Nalp3 inflammasome","volume":"119","author":"Imaeda AB","year":"2009","journal-title":"J Clin Invest"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.21568"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(01)00284-3"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.5.1541"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1462399402004155"},{"key":"e_1_2_5_12_1","first-page":"481","article-title":"Expression patterns of leukocyte adhesion ligand molecules on human liver endothelia. 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