{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:37:03Z","timestamp":1761597423265,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,5,26]],"date-time":"2013-05-26T00:00:00Z","timestamp":1369526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AI-51445","R01 DK 50574"],"award-info":[{"award-number":["AI-51445","R01 DK 50574"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Institute of Aging"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>The liver's response to injury is fibrosis, and when chronic, cirrhosis. Age is a critical factor impacting many immune\u2010mediated processes, potentially including the liver's wounding response to injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The effects of age on acute and chronic liver injury were evaluated using a carbon tetrachloride model in mice. Lymphocyte and macrophage populations were assessed by flow cytometry and immunohistochemical analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Acute liver injury was greater in 18\u2010month\u2010old (old) mice than in 9\u2010month\u2010old (middle\u2010aged) mice as judged by changes in aminotransferases. Similarly, livers of 18\u2010month\u2010old mice had a significantly greater fibrogenic response to injury than did livers of 9\u2010month\u2010old mice after chronic injury (assessed by col1\u03b11 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression, morphometric analysis and hydroxyproline measurement). Interestingly, livers from young mice (6\u00a0weeks old) also exhibited an increase in fibrogenesis compared to 9\u2010month\u2010old mice, albeit not to the same degree as in old mice. Consistent with a role for macrophages in fibrogenesis, the number of liver macrophages in young and 9\u2010month\u2010old mice increased, while in chronically injured livers of 18\u2010month\u2010old mice, the number of macrophages was reduced, and was less than in the livers of young and 9\u2010month\u2010old injured livers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data indicate that the fibrogenic response to injury varies substantially with age, and moreover that macrophage recruitment and dynamics may be an important component in differential age\u2010associated fibrotic disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12202","type":"journal-article","created":{"date-parts":[[2013,4,20]],"date-time":"2013-04-20T08:12:38Z","timestamp":1366445558000},"page":"1175-1186","source":"Crossref","is-referenced-by-count":29,"title":["Association of age\u2010dependent liver injury and fibrosis with immune cell populations"],"prefix":"10.1111","volume":"33","author":[{"given":"Bradley H.","family":"Collins","sequence":"first","affiliation":[{"name":"Department of Surgery Duke University Medical Center Durham NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoie E.","family":"Holzknecht","sequence":"additional","affiliation":[{"name":"Department of Surgery Duke University Medical Center Durham NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kellie A.","family":"Lynn","sequence":"additional","affiliation":[{"name":"Department of Surgery Duke University Medical Center Durham NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory D.","family":"Sempowski","sequence":"additional","affiliation":[{"name":"Department of Medicine Duke Human Vaccine Institute Duke University School of Medicine Durham NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine C.","family":"Smith","sequence":"additional","affiliation":[{"name":"Liver Center Laboratory and Department of Medicine Duke University Medical Center Durham NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Medical University of South Carolina Charleston SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Parker","sequence":"additional","affiliation":[{"name":"Department of Surgery Duke University Medical Center Durham NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don C.","family":"Rockey","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Medical University of South Carolina Charleston SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000211670"},{"key":"e_1_2_7_3_1","first-page":"73","article-title":"Carbon tetrachloride cirrhosis in rats. 1. 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