{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:29:51Z","timestamp":1790746191989,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,5,20]],"date-time":"2013-05-20T00:00:00Z","timestamp":1369008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology of Japan","doi-asserted-by":"crossref","award":["23249043"],"award-info":[{"award-number":["23249043"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Health, Labour and Welfare of Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Neutrophils infiltrate the livers of patients with nonalcoholic steatohepatitis (\n                      <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>\n                      ). Human neutrophil peptides (\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      s) induce cytokine and chemokine production under inflammatory conditions, which may contribute to the progression of\n                      <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>\n                      . In this study, we focused on the effects of\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 on hepatic steatosis and fibrosis in a mouse model of\n                      <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>\n                      induced by a choline\u2010deficient, L\u2010amino acid\u2013defined (\n                      <jats:styled-content style=\"fixed-case\">CDAA<\/jats:styled-content>\n                      ) diet.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials &amp; Methods<\/jats:title>\n                    <jats:p>\n                      We generated transgenic mice expressing\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 under the control of a \u03b2\u2010actin\u2013based promoter.\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 transgenic and wild\u2010type C57\n                      <jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\n                      \/6N mice were fed a\n                      <jats:styled-content style=\"fixed-case\">CDAA<\/jats:styled-content>\n                      diet for 16\u00a0weeks to induce hepatic steatosis and fibrosis. Serological and histological features were examined, and the effects of\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 on hepatic stellate cell lines were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 transgenic and wild\u2010type mice fed the\n                      <jats:styled-content style=\"fixed-case\">CDAA<\/jats:styled-content>\n                      diet showed no significant differences in serum alanine aminotransferase levels or the degree of hepatic steatosis based on Oil red O staining and hepatic triglyceride content. In contrast, Sirius Red and Azan staining showed significantly more severe hepatic fibrosis in\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 transgenic mice compared with wild\u2010type mice. In addition, significantly more \u03b1\u2010smooth muscle actin\u2013positive hepatic stellate cells were observed in the transgenic mice than in the wild\u2010type mice. Finally, the proliferation of the\n                      <jats:styled-content style=\"fixed-case\">LI<\/jats:styled-content>\n                      90 hepatic stellate cell line increased in response to\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our data indicate that\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 enhances hepatic fibrosis in fatty liver by inducing hepatic stellate cell proliferation. Thus, neutrophil\u2010derived\n                      <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>\n                      \u20101 may contribute to the progression of\n                      <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.12203","type":"journal-article","created":{"date-parts":[[2013,4,22]],"date-time":"2013-04-22T13:23:44Z","timestamp":1366637024000},"page":"1549-1556","source":"Crossref","is-referenced-by-count":53,"title":["Transgenic expression of human neutrophil peptide\u20101 enhances hepatic fibrosis in mice fed a choline\u2010deficient, L\u2010amino acid\u2013defined diet"],"prefix":"10.1111","volume":"33","author":[{"given":"Rie","family":"Ibusuki","sequence":"first","affiliation":[{"name":"Digestive and Lifestyle Diseases Department of Human and Environmental Sciences Kagoshima University Graduate School of 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