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In this nationwide cross\u2010sectional study, we aimed to investigate the epidemiology and clinical significance of HBV<\/jats:styled-content> genotype and qHBsAg<\/jats:styled-content> in patients with chronic hepatitis B (CHB<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Six hundred and thirty patients with CHB<\/jats:styled-content> were seen in four urban tertiary referral centres in Canada. HBV<\/jats:styled-content> genotype was determined by line probe assay (INNO\u2010LIPA) and HBV DNA<\/jats:styled-content> quantified by commercial PCR<\/jats:styled-content> (Roche TaqMan, sensitivity <55\u00a0IU\/ml or AMPLICOR, sensitivity <60\u00a0IU\/ml). Titres of qHBsAg<\/jats:styled-content> were determined by an in\u2010house assay based on the WHO<\/jats:styled-content> standard (calibration range 0.24\u201362.5\u00a0IU\/ml).<\/jats:p><\/jats:sec>Results<\/jats:title>In 630 patients (57% male, 69% Asian, median age 42\u00a0years), 21% were hepatitis B e antigen positive and the median alanine aminotransferase was 29\u00a0U\/L. The HBV<\/jats:styled-content> genotype distribution was A (16%), B (29%), C (31%), D (16%), E (6%). HBV<\/jats:styled-content> genotype was strongly associated with ethnicity, but neither genotype nor qHBsAg<\/jats:styled-content> correlated with the degree of fibrosis. In the treatment\u2010na\u00efve patients, the baseline qHBsAg<\/jats:styled-content> levels correlated with HBV DNA<\/jats:styled-content> (r<\/jats:italic>\u00a0=\u00a00.2517, P\u00a0<\u00a0<\/jats:italic>0.0008). The median qHBsAg<\/jats:styled-content> levels were lowest in patients with genotype B (P\u00a0<\u00a0<\/jats:italic>0.0001), but no significant correlation was noted with all other HBV<\/jats:styled-content> genotypes.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>In this large North American HBV<\/jats:styled-content> epidemiological study, genotypes B and C were the most common; however, all genotypes (A\u2013E) were observed with varied distribution nationwide. Baseline qHBsAg<\/jats:styled-content> significantly correlated with HBV DNA<\/jats:styled-content> and with HBV<\/jats:styled-content> genotype B, but not with liver fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12222","type":"journal-article","created":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T05:44:07Z","timestamp":1368769447000},"page":"1363-1369","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of hepatitis B virus genotypes and quantitative hepatitis B surface antigen titres in North American tertiary referral liver centres"],"prefix":"10.1111","volume":"33","author":[{"given":"Stephen E.","family":"Congly","sequence":"first","affiliation":[{"name":"Liver Unit Division of Gastroenterology and Hepatology University of Calgary Calgary AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Wong","sequence":"additional","affiliation":[{"name":"McGill University Health Centre Royal Victoria Hospital 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