{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:30:52Z","timestamp":1780511452731,"version":"3.54.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T00:00:00Z","timestamp":1376524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"crossref","award":["FIS 07\/0157"],"award-info":[{"award-number":["FIS 07\/0157"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The current methods available for screening and detecting hepatocellular carcinoma (<jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>) have insufficient sensitivity and specificity, and only a low percentage of diagnosis of small tumours is based on these assays. Because <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> is usually asymptomatic at potentially curative stages, identification of biomarkers for the early detection of <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> is essential to improve patient survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study was to identify candidate markers for <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> development in the plasma from hepatitis C virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>)\u2010infected cirrhotic patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared protein expression profiles of plasma samples from <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected cirrhotic patients with and without <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>, using two\u2010dimensional fluorescence difference gel electrophoresis (2\u2010D <jats:styled-content style=\"fixed-case\">DIGE<\/jats:styled-content>) coupled with <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> mass spectrometry. The 2\u2010D <jats:styled-content style=\"fixed-case\">DIGE<\/jats:styled-content> results were analysed statistically using <jats:italic>Decyder<\/jats:italic>\u2122 software, and verified by western blot and enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the plasma of <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> patients, we observed decreased expression of complement component 9, ficolin\u20103 (<jats:styled-content style=\"fixed-case\">FCN<\/jats:styled-content>3), serum amyloid P component (<jats:styled-content style=\"fixed-case\">SAP<\/jats:styled-content>), fibrinogen\u2010gamma and immunoglobulin gamma\u20101 chain, and increased expression of vitronectin (<jats:styled-content style=\"fixed-case\">VTN<\/jats:styled-content>) and galectin\u20103 binding protein (G3<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>) by <jats:styled-content style=\"fixed-case\">DIGE<\/jats:styled-content> analysis. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> confirmed <jats:styled-content style=\"fixed-case\">DIGE<\/jats:styled-content> results for <jats:styled-content style=\"fixed-case\">VTN<\/jats:styled-content> and G3<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content> but not for <jats:styled-content style=\"fixed-case\">SAP<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">FCN<\/jats:styled-content>3 in a larger patient population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The proteins <jats:styled-content style=\"fixed-case\">VTN<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SAP<\/jats:styled-content> are candidate biomarkers for <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> development in <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected cirrhotic patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12277","type":"journal-article","created":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T04:45:05Z","timestamp":1374468305000},"page":"438-446","source":"Crossref","is-referenced-by-count":37,"title":["Identification of candidate biomarkers for hepatocellular carcinoma in plasma of <scp>HCV<\/scp>\u2010infected cirrhotic patients by 2\u2010D <scp>DIGE<\/scp>"],"prefix":"10.1111","volume":"34","author":[{"given":"Gustavo","family":"Ferr\u00edn","sequence":"first","affiliation":[{"name":"Maim\u00f3nides Institute for Biomedical Research in C\u00f3rdoba (IMBIC) Reina Sof\u00eda University Hospital University of C\u00f3rdoba C\u00f3rdoba Spain"},{"name":"Networked Biomedical Research Center, Hepatic and Digestive Diseases (CIBERehd) Barcelona 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