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Oxidative stress is an important component in the pathophysiology of <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>. Several enzymatic antioxidant mechanisms protect the liver from oxidative injury. Examination of the expression of these enzymes in <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content> livers may provide insight on the roles for these antioxidant mechanisms in the pathophysiology of <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of catalase, glutathione peroxidase 1 (GPX1), glutathione reductase (GSR), paraoxonase 1 (PON1) and other reactive oxygen species\u2010related genes was evaluated by microarray and quantitative real\u2010time PCR analyses. The PON1 protein levels were evaluated in liver and serum by Western blot analyses. Serum enzymatic activities of GPX, GSR and PON1 (paraoxonase and arylesterase activities) were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NASH livers exhibited elevated <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of catalase and PON1, but not GPX1 or GSR. No difference in serum GPX or GSR activity was detected between NASH patients and controls. Elevated expression of PON1 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein was detected in NASH livers, but serum PON1 protein and activities were not elevated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Elevated expression of catalase and <jats:styled-content style=\"fixed-case\">PON<\/jats:styled-content>1 suggests protective roles for these antioxidants in <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content> livers. Given the importance of oxidative stress in the pathophysiology of <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>, future studies focusing on these enzymes could identify important targets for therapeutic or preventive interventions for <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12308","type":"journal-article","created":{"date-parts":[[2013,8,21]],"date-time":"2013-08-21T05:30:46Z","timestamp":1377063046000},"page":"110-117","source":"Crossref","is-referenced-by-count":33,"title":["Paraoxonase 1 and oxidative stress in paediatric non\u2010alcoholic steatohepatitis"],"prefix":"10.1111","volume":"34","author":[{"given":"Sonal","family":"Desai","sequence":"first","affiliation":[{"name":"Women and Children's Hospital of Buffalo Department of Pediatrics the State University of New York Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan S.","family":"Baker","sequence":"additional","affiliation":[{"name":"Women and Children's Hospital of Buffalo Department of Pediatrics the State University of New York Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wensheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Women and Children's Hospital of Buffalo Department of Pediatrics the State University of New York Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana A.","family":"Moya","sequence":"additional","affiliation":[{"name":"Women and Children's Hospital of Buffalo Department of Pediatrics the State University of New York Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"Browne","sequence":"additional","affiliation":[{"name":"Department of Biotechnical and Clinical Laboratory Sciences the State University of New York at Buffalo Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucy","family":"Mastrandrea","sequence":"additional","affiliation":[{"name":"Women and Children's Hospital of Buffalo Department of Pediatrics the State University of New York Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D.","family":"Baker","sequence":"additional","affiliation":[{"name":"Women and Children's Hospital of Buffalo Department of Pediatrics the State University of New York Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Women and Children's Hospital of Buffalo Department of Pediatrics the State University of New York Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2012.40"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2011.04724.x"},{"key":"e_1_2_7_4_1","first-page":"434","article-title":"Nonalcoholic steatohepatitis: mayo Clinic experiences with a hitherto unnamed disease","volume":"55","author":"Ludwig J","year":"1980","journal-title":"Mayo Clinic Proc"},{"key":"e_1_2_7_5_1","first-page":"151","article-title":"Nonalcoholic fatty liver disease in children","volume":"14","author":"Mencin AA","year":"2011","journal-title":"Curr Opin Clin Nutr Metab Care"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70599-2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"G1135","DOI":"10.1152\/ajpgi.2001.281.5.G1135","article-title":"Nonalcoholic steatosis and steatohepatitis. 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