{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:43:09Z","timestamp":1762144989462,"version":"3.37.3"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,10,2]],"date-time":"2013-10-02T00:00:00Z","timestamp":1380672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"crossref","award":["20020110100030"],"award-info":[{"award-number":["20020110100030"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"crossref","award":["11220110100375"],"award-info":[{"award-number":["11220110100375"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>Intrahepatic cholestasis of pregnancy is a high\u2010risk liver disease given the eventual deleterious consequences that may occur in the foetus. It is accepted that the abnormal accumulation of hydrophobic bile acids in maternal serum are responsible for the disease development. Hydrophobic bile acids induce oxidative stress and apoptosis leading to the damage of the hepatic parenchyma and eventually extrahepatic tissues. As coenzyme Q (CoQ) is considered an early marker of oxidative stress in this study, we sought to assess CoQ levels, bile acid profile and oxidative stress status in intrahepatic cholestasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CoQ, vitamin E and malondialdehyde were measured in plasma and\/or tissues by <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> method whereas serum bile acids by capillary electrophoresis in rats with ethinyl estradiol\u2010induced cholestasis and women with pregnancy cholestasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CoQ and vitamin E plasma levels were diminished in both rats and women with intrahepatic cholestasis. Furthermore, reduced CoQ was also found in muscle and brain of cholestatic rats but no changes were observed in heart or liver. In addition, a positive correlation between CoQ and ursodeoxycholic\/lithocholic acid ratio was found in intrahepatic cholestasis suggesting that increased plasma lithocholic acid may be intimately related to CoQ depletion in blood and tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Significant CoQ and vitamin E depletion occur in both animals and humans with intrahepatic cholestasis likely as the result of increased hydrophobic bile acids known to produce significant oxidative stress. Present findings further suggest that antioxidant supplementation complementary to traditional treatment may improve cholestasis outcome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12323","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T11:51:23Z","timestamp":1378900283000},"page":"1040-1048","source":"Crossref","is-referenced-by-count":8,"title":["Coenzyme Q in pregnant women and rats with intrahepatic cholestasis"],"prefix":"10.1111","volume":"34","author":[{"given":"Manuela R.","family":"Martinefski","sequence":"first","affiliation":[{"name":"Analytical Chemistry School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario D.","family":"Contin","sequence":"additional","affiliation":[{"name":"Analytical Chemistry School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myrian R.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Pathophysiology\u2010INIGEM School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estefan\u00eda M.","family":"Ger\u00e9z","sequence":"additional","affiliation":[{"name":"Physical Chemistry\u2010IBIMOL School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica L.","family":"Galleano","sequence":"additional","affiliation":[{"name":"Physical Chemistry\u2010IBIMOL School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia E.","family":"Lucangioli","sequence":"additional","affiliation":[{"name":"Drug Quality School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliana G.","family":"Bianciotti","sequence":"additional","affiliation":[{"name":"Pathophysiology\u2010INIGEM School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria P.","family":"Tripodi","sequence":"additional","affiliation":[{"name":"Drug Quality School of Pharmacy and Biochemistry Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-5576(01)00144-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.6014.870"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(00)80139-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20336"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.804"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2007.04.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2001.10719063"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.5.1646"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000078382"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1072"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/500092"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2007.02.008"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2008.02318.x"},{"key":"e_1_2_7_15_1","first-page":"71","article-title":"Coenzyme Q10: is there a clinical role and a case for measurement?","volume":"29","author":"Molineux S","year":"2008","journal-title":"Clin Bioche Rev"},{"key":"e_1_2_7_16_1","first-page":"1399","article-title":"Lipid peroxidation, DNA damage and coenzyme Q10 in lung cancer patients\u2013markers for risk assessment?","volume":"12","author":"Cobanoglu U","year":"2011","journal-title":"Asian Pac J Cancer Prev"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2009.08.008"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2010.08.010"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/S1665-2681(19)32126-X","article-title":"Cholestasis: human disease and experimental animal models","volume":"2","author":"Rodr\u00edguez Garay E","year":"2003","journal-title":"Ann Hepatol"},{"key":"e_1_2_7_20_1","first-page":"365","article-title":"Molecular alterations of canalicular transport systems in experimental models of cholestasis: possible functional correlations","volume":"70","author":"Trauner M","year":"1997","journal-title":"Yale J Biol Med"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2003.12.002"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2011.591028"},{"key":"e_1_2_7_23_1","first-page":"273","article-title":"Sistema cromatogr\u00e1fico miniaturizado para la determinaci\u00f3n de coenzima Q10 en plasma, m\u00fasculo y plaquetas","volume":"45","author":"Contin M","year":"2011","journal-title":"Acta Bioqu\u00edm Cl\u00edn latinoam"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.07.009"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/14.4.946"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00922-4"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00916-4"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20050302"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1880321"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200104000-00014"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.39.3.475"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-9877(86)90137-4"},{"key":"e_1_2_7_33_1","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1016\/S0022-2275(20)42614-8","article-title":"Bile acid structure\u2010activity relationship: evaluation of bile acid lipophilicity using 1\u2010octano\/water partition coefficient and reverse phase HPLC","volume":"31","author":"Roda A","year":"1990","journal-title":"J Lipid Res"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.02.028"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.10.020"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2006.05.015"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.106.009308"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.113209"},{"key":"e_1_2_7_39_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S0016-5085(19)33531-0","article-title":"Studies on the mechanism of the ethynylestradiol impairment of bile flow and bile salt excretion in the rat","volume":"61","author":"Gumuccio JJ","year":"1971","journal-title":"Gastroenterology"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(02)00225-9"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2006.06.016"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1665-2681(19)31884-8"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1253228"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2010.06592.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2800373"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840080228"},{"key":"e_1_2_7_47_1","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1016\/S0022-2275(20)38222-5","article-title":"Regulation of hepatic cholesterol and lipoprotein metabolism in ethinyl estradiol\u2010treated rats","volume":"30","author":"Erickson SK","year":"1989","journal-title":"J Lipid Res"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114552"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64849-3"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1159\/000087586"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1159\/000264313"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2000.00729.x"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2008.008130"}],"container-title":["Liver International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fliv.12323","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fliv.12323","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/liv.12323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T13:13:30Z","timestamp":1695474810000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/liv.12323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,2]]},"references-count":52,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/liv.12323"],"URL":"https:\/\/doi.org\/10.1111\/liv.12323","archive":["Portico"],"relation":{},"ISSN":["1478-3223","1478-3231"],"issn-type":[{"type":"print","value":"1478-3223"},{"type":"electronic","value":"1478-3231"}],"subject":[],"published":{"date-parts":[[2013,10,2]]}}}