{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:09:09Z","timestamp":1791295749576,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T00:00:00Z","timestamp":1381881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005297","name":"Medical University of Bia\u0142ystok","doi-asserted-by":"crossref","award":["114-18872"],"award-info":[{"award-number":["114-18872"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005297","name":"Medical University of Bia\u0142ystok","doi-asserted-by":"crossref","award":["123-18805"],"award-info":[{"award-number":["123-18805"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>Nonalcoholic fatty liver disease (<jats:styled-content style=\"fixed-case\">NAFLD<\/jats:styled-content>) is an insulin resistance\u2010related hepatic disorder which can transform to cirrhosis. Insulin resistance deregulates hepatic lipid metabolism, leading to accumulation of cytotoxic lipids including ceramide and diacylglycerols. Myriocin, obtained from fungi traditionally used in Chinese medicine in an effort to attain eternal youth, is a potent pharmacological inhibitor of ceramide <jats:italic>de novo<\/jats:italic> synthesis. We examined whether inhibition of ceramide <jats:italic>de novo<\/jats:italic> synthesis with myriocin ameliorate hepatic lipid accumulation and reverse <jats:styled-content style=\"fixed-case\">NAFLD<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The experiment was carried out on male Wistar rats. The animals were divided into four groups: (i) control group, fed standard rodent diet, (ii) group, fed standard diet also treated with myriocin for 7 days, (iii) group, fed high\u2010fat diet for 5\u00a0weeks, (iv) group, fed high\u2010fat diet and treated with myriocin. In liver samples sphingolipids: ceramide, sphingosine and sphingosine\u20101\u2010phosphatate and neutral lipids, such as diacylglycerols and triacylglycerols were measured. In peripheral blood samples, glucose and insulin levels and aminotransferases activities were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High\u2010fat diet feeding caused <jats:styled-content style=\"fixed-case\">NAFLD<\/jats:styled-content>, confirmed by histological assessment, with increased hepatic lipids accumulation and whole\u2010body insulin resistance. After treating with inhibitor of ceramide <jats:italic>de novo<\/jats:italic> synthesis, decrease in hepatic ceramide and other toxic lipids were noticed. Moreover, histological analysis of liver samples revealed that inhibition of ceramide <jats:italic>de novo<\/jats:italic> synthesis reduced hepatic steatosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Inhibition of ceramide <jats:italic>de novo<\/jats:italic> synthesis reduced hepatic lipid accumulation in rats with <jats:styled-content style=\"fixed-case\">NAFLD<\/jats:styled-content>, this led to amelioration of hepatic steatosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12331","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:02:42Z","timestamp":1381539762000},"page":"1074-1083","source":"Crossref","is-referenced-by-count":124,"title":["Inhibition of ceramide <i>de novo<\/i> synthesis reduces liver lipid accumulation in rats with nonalcoholic fatty liver disease"],"prefix":"10.1111","volume":"34","author":[{"given":"Krzysztof","family":"Kurek","sequence":"first","affiliation":[{"name":"Department of Physiology Medical University of Bialystok Bia\u0142ystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika M.","family":"Piotrowska","sequence":"additional","affiliation":[{"name":"Department of Public Health Medical University of Bialystok Bia\u0142ystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrycja","family":"Wiesio\u0142ek\u2010Kurek","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical University of Bialystok Bia\u0142ystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart\u0142omiej","family":"\u0141ukaszuk","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical University of Bialystok Bia\u0142ystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Chabowski","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical University of Bialystok Bia\u0142ystok 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