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Serum fetuin\u2010A concentration is associated with nonalcoholic fatty liver disease (NAFLD) and cardiovascular disease. However, the usefulness of serum fetuin\u2010A as a predictive fibrosis biomarker in NAFLD patients remains unclear. In this study, we investigated the relationship between circulating fetuin\u2010A levels and fibrosis\u2010related markers [platelet count, NAFLD fibrosis score and carotid intima media thickness (IMT)] in subjects with NAFLD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 295 subjects (male, 164; female, 131) who received medical health check\u2010ups were enrolled in this study. NAFLD was diagnosed using abdominal ultrasonography. Serum fetuin\u2010A was measured by ELISA. IMT was assessed using a high\u2010resolution ultrasound scanner. Using recombinant human fetuin\u2010A, we investigated the effects of fetuin\u2010A on hepatic stellate cells, which play a pivotal role in the process of hepatic fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum fetuin\u2010A concentration was significantly correlated with platelet count (<jats:italic>R<\/jats:italic>\u00a0=\u00a00.19, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01), NAFLD fibrosis score (<jats:italic>R<\/jats:italic>\u00a0=\u00a0\u22120.25, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and mean IMT (<jats:italic>R<\/jats:italic>\u00a0=\u00a0\u22120.22, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01). Multivariate analyses revealed that the fetuin\u2010A concentration is a significant and independent determinant of platelet count, NAFLD fibrosis score and mean IMT. Recombinant fetuin\u2010A suppressed TGF\u2010\u03b21 signalling and fibrosis\u2010related gene expression and increased the expression of TGF\u2010\u03b21 pseudoreceptor bone morphogenic protein and activin membrane\u2010bound inhibitor (BAMBI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Serum fetuin\u2010A level is associated with liver\/vessel fibrosis\u2010related markers in NAFLD patients. Circulating fetuin\u2010A could be a useful serum biomarker for predicting liver and vascular fibrosis progression in NAFLD patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12478","type":"journal-article","created":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T11:51:19Z","timestamp":1391255479000},"page":"925-935","source":"Crossref","is-referenced-by-count":61,"title":["Fetuin\u2010A negatively correlates with liver and vascular fibrosis in nonalcoholic fatty liver disease subjects"],"prefix":"10.1111","volume":"35","author":[{"given":"Motoya","family":"Sato","sequence":"first","affiliation":[{"name":"Department of Molecular Biochemistry &amp; Clinical Investigation Osaka University Graduate School of Medicine Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Kamada","sequence":"additional","affiliation":[{"name":"Department of Molecular Biochemistry &amp; Clinical Investigation Osaka University Graduate School of Medicine Suita Osaka Japan"},{"name":"Department of Gastroenterology and Hepatology Osaka University Graduate School of Medicine Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Molecular Biochemistry &amp; Clinical Investigation Osaka University Graduate School of Medicine Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiho","family":"Kida","sequence":"additional","affiliation":[{"name":"Department of Molecular Biochemistry &amp; Clinical Investigation Osaka University Graduate School of Medicine Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Ohara","sequence":"additional","affiliation":[{"name":"Department of Molecular Biochemistry &amp; Clinical Investigation Osaka University Graduate School of Medicine Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironobu","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Molecular Biochemistry &amp; Clinical Investigation Osaka University Graduate School of Medicine Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maaya","family":"Akita","sequence":"additional","affiliation":[{"name":"Department of Molecular Biochemistry &amp; 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