{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T12:24:09Z","timestamp":1768911849639,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,7,5]],"date-time":"2014-07-05T00:00:00Z","timestamp":1404518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SFB TRR57"],"award-info":[{"award-number":["SFB TRR57"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"H. J. & W. Hector Stiftung"},{"name":"Capital Region of Denmark Foundation of Health Research"},{"name":"Hvidovre Hospital Foundation for Liver Diseases"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>Patients with cirrhosis display hypocontractility of splanchnic vessels because of dysregulation of vasoactive proteins, such as decreased effect of RhoA\/ROCK and increased activity of \u03b2\u2010Arrestin\u20102 and <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content>. However, it is unknown whether the dysregulation of vasoactive proteins is displayed in other vessels. We investigated whether expression of vasoactive proteins can be evaluated in gastric mucosa vessels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Biopsies from the gastric mucosa of 111 patients with cirrhosis were collected at three different centres and from 13 controls. Forty\u2010nine patients had received <jats:styled-content style=\"fixed-case\">TIPS<\/jats:styled-content>. Portal pressure gradient was measured in 49 patients with <jats:styled-content style=\"fixed-case\">TIPS<\/jats:styled-content> and in 16 patients without <jats:styled-content style=\"fixed-case\">TIPS<\/jats:styled-content>. Biopsies from the antrum were conserved in formaldehyde for immunohistochemistry or shock\u2010frozen for <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mucosal transcription of vascular markers (\u03b1SMA, CD31) was higher in cirrhotic patients than controls, which was confirmed by immunohistochemistry. On average, relative mucosal levels of RhoA and ROCK were lower, while \u03b2\u2010Arrestin\u20102 levels were higher in cirrhotic patients compared to controls. Transcriptional levels of <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content> increased with presence of ascites and grade of oesophageal varices. Patients with TIPS showed less pronounced markers of vascular dysfunction in gastric mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first evidence that the expression of vasoactive proteins in mucosa from the gastric antrum of patients with cirrhosis reflects their vascular dysfunction and possibly changes after therapeutic interventions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12613","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T04:43:06Z","timestamp":1402375386000},"page":"1393-1402","source":"Crossref","is-referenced-by-count":13,"title":["Expression of vasoactive proteins in gastric antral mucosa reflects vascular dysfunction in patients with cirrhosis and portal hypertension"],"prefix":"10.1111","volume":"35","author":[{"given":"Jonel","family":"Trebicka","sequence":"first","affiliation":[{"name":"Department of Internal Medicine I University of Bonn Bonn Germany"},{"name":"Gastrounit Medical Division Hvidovre Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyrus","family":"Wix","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I University of Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"von Heydebrand","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I University of Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanishka","family":"Hittatiya","sequence":"additional","affiliation":[{"name":"Institute for Pathology University of Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Reiberger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III Division of Gastroenterology &amp; Hepatology Vienna Hepatic Hemodynamic Lab Medical University of Vienna Vienna 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