{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:39:56Z","timestamp":1782362396091,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T00:00:00Z","timestamp":1409097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2015,2]]},"abstract":"Abstract<\/jats:title>Background & Aims<\/jats:title>Antibodies (Abs) to soluble liver antigen\/liver pancreas (anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content>) are considered markers of worse prognosis and outcome in patients with autoimmune hepatitis (AIH<\/jats:styled-content>) although this assumption has recently been attributed to their frequent co\u2010expression with Abs against Ro52 (anti\u2010Ro52). To assess the clinical significance of anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content> Abs alone or in combination with anti\u2010Ro52 in AIH<\/jats:styled-content> patients and determine the immunodominant Ro52 epitopes according to the anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content> status.<\/jats:p><\/jats:sec>Methods<\/jats:title>Twenty\u2010three anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content>\u2010positive and 106 anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content>\u2010negative AIH<\/jats:styled-content> patients were included. Anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content> were determined by ELISA<\/jats:styled-content> using recombinant antigen, and confirmed by immunoblot using cytosolic rat liver fraction or HuH\u20107 extract. Anti\u2010Ro52 Abs were determined by ELISA<\/jats:styled-content> using recombinant antigen. Epitope mapping was assessed by ELISA<\/jats:styled-content> using overlapping peptides covering the whole Ro52 protein in 26 AIH<\/jats:styled-content> patients and 12 patients with Sj\u00f6gren's syndrome.<\/jats:p><\/jats:sec>Results<\/jats:title>Anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content> positivity was not associated with the clinical, laboratory or histological characteristics of AIH<\/jats:styled-content> patients. Treatment response, corticosteroid withdrawal, relapse after stopping treatment and outcome, were not associated with the presence of anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content>, anti\u2010Ro52 or double reactivity. Moreover, Ro52 epitope mapping revealed new epitopes unique for AIH<\/jats:styled-content> and independent from anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content> positivity.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Neither anti\u2010SLA<\/jats:styled-content>\/LP<\/jats:styled-content> nor anti\u2010Ro52 Abs or their combination could specify a distinct group of AIH<\/jats:styled-content> patients in terms of clinical characteristics, treatment response and outcome. Further studies are needed to clarify whether the newly discovered immunodominant epitopes of Ro52 antigen which were associated specifically with AIH<\/jats:styled-content> have any clinical or pathogenetic significance in AIH<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12658","type":"journal-article","created":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T16:54:23Z","timestamp":1407776063000},"page":"660-672","source":"Crossref","is-referenced-by-count":46,"title":["Anti\u2010SLA<\/scp>\/LP<\/scp> alone or in combination with anti\u2010Ro52 and fine specificity of anti\u2010Ro52 antibodies in patients with autoimmune hepatitis"],"prefix":"10.1111","volume":"35","author":[{"given":"Kalliopi","family":"Zachou","sequence":"first","affiliation":[{"name":"Department of Medicine and Research Laboratory of Internal Medicine School of Medicine University of Thessaly Larissa 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Medicine Aristotelian University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menelaos N.","family":"Manoussakis","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology School of Medicine National University of Athens Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Renaudineau","sequence":"additional","affiliation":[{"name":"Research Unit EA2216 Immunology and Pathology H\u00f4pital Morvan et Cavale blanche Universit\u00e9 Europ\u00e9enne de Bretagne France and Universit\u00e9 de Brest IFR 148 ScInBioS CHU Brest Brest France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios P.","family":"Bogdanos","sequence":"additional","affiliation":[{"name":"Division of Rheumatology School of Medicine University of Thessaly Larissa Greece"},{"name":"Institute of Liver Studies King's College London School of Medicine London 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