{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:43:29Z","timestamp":1790372609923,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,22]],"date-time":"2014-09-22T00:00:00Z","timestamp":1411344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["K24 DK069290A"],"award-info":[{"award-number":["K24 DK069290A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background &amp; Aims<\/jats:title>\n                    <jats:p>\n                      A greater understanding of cholestatic disease is necessary to advance diagnostic tools and therapeutic options for conditions such as primary biliary cirrhosis (\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      ) and primary sclerosing cholangitis (\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      ). The purpose of this study was to determine and compare the serum metabolomes of patients with\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a018) or\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a021) and healthy controls (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a010) and to identify metabolites that may differentiate these two cholestatic diseases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      Using a mass spectrometry\u2010based, non\u2010targeted biochemical profiling approach, we identified 420 serum metabolites, 101 that differed significantly (\n                      <jats:italic>P\u00a0<\/jats:italic>\n                      \u2264\n                      <jats:italic>\u00a0<\/jats:italic>\n                      0.05) between\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      and control groups, 115 that differed significantly between\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      and control groups, and 56 that differed significantly between\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      groups. Random forest classification analysis was able to distinguish patients with\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      or\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      with 95% accuracy with selected biochemicals reflective of protein and amino acid metabolism identified as the major contributors. Metabolites related to bile acid metabolism, lipid metabolism, inflammation, and oxidative stress\/lipid peroxidation were also identified as differing significantly when comparing the disease groups and controls, with some of these pathways differentially affected in the\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      In this study, we identified novel metabolic changes associated with cholestatic disease that were both consistent and different between\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      . Validation studies in larger patient cohorts are required to determine the utility of these biochemical markers for diagnosis and therapeutic monitoring of patients with\n                      <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.12680","type":"journal-article","created":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T11:12:04Z","timestamp":1409656324000},"page":"263-274","source":"Crossref","is-referenced-by-count":71,"title":["Serum metabolic signatures of primary biliary cirrhosis and primary sclerosing cholangitis"],"prefix":"10.1111","volume":"35","author":[{"given":"Lauren N","family":"Bell","sequence":"first","affiliation":[{"name":"Metabolon Inc. 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