{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:40:54Z","timestamp":1788446454322,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,2,6]],"date-time":"2015-02-06T00:00:00Z","timestamp":1423180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Foundation for Medical Research of the Tri-Service General Hospital","award":["TSGH-C101-066"],"award-info":[{"award-number":["TSGH-C101-066"]}]},{"name":"Foundation for Medical Research of the Tri-Service General Hospital","award":["TSGH-C102-063"],"award-info":[{"award-number":["TSGH-C102-063"]}]},{"name":"National Defense Medical Center","award":["DOD-100-I-27"],"award-info":[{"award-number":["DOD-100-I-27"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2015,8]]},"abstract":"Abstract<\/jats:title>\n \n Background & Aims<\/jats:title>\n \n Gilbert's syndrome causes pharmacological variation in drug glucuronidation and unexpected toxicity from therapeutic agents. The two common genotypes of Gilbert's syndrome are a dinucleotide polymorphism (\n TA<\/jats:styled-content>\n )\n 7<\/jats:sub>\n in\n TATA<\/jats:styled-content>\n \u2010Box as well as the 211G>A mutation in the coding exon 1, particularly in Asians, of human\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n gene. In this study, we aimed to establish an effective method to detect the 211G>A mutation.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The coding exon 1 sequence of human\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n gene was analysed by Vector\n NTI<\/jats:styled-content>\n software. The 211G>A mutation in the coding exon\u00a01 of\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n gene was determined by restriction fragment length polymorphism (\n RFLP<\/jats:styled-content>\n ) method. Serum total bilirubin level was measured as well.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A newly identified Bsm\n BI<\/jats:styled-content>\n site was located in the coding exon 1 of\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n gene. The 211G>A mutation in the coding exon 1 of\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n gene was determined by\n DNA RFLP<\/jats:styled-content>\n . Furthermore, we reported our present work on genetic analysis of mutations of\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n gene, and the correlation of\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n mutations with serum total bilirubin levels in Taiwanese population. The results showed that 15 subjects carried 211G>A mutation in 23 subjects related with Gilbert's syndrome. The homozygous 211G>A mutant as well as simultaneously heterozygous mutants both in\n TATA<\/jats:styled-content>\n \u2010Box and 211G>A significantly increased the risk of Gilbert's syndrome similar to subjects carrying homozygous\n TATA<\/jats:styled-content>\n \u2010Box mutant.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Bsm\n BI RFLP<\/jats:styled-content>\n is an effective method to detect 211G>A mutation in the coding exon 1 of\n \n UGT<\/jats:styled-content>\n 1A1\n <\/jats:italic>\n gene. The common 211G>A mutation is one of the causes of Gilbert's syndrome in Taiwanese population.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/liv.12785","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T08:24:14Z","timestamp":1421915054000},"page":"2050-2056","source":"Crossref","is-referenced-by-count":10,"title":["Restriction fragment length polymorphism effectively identifies exon 1 mutation of\n UGT<\/scp>\n 1A1 gene in patients with Gilbert's Syndrome"],"prefix":"10.1111","volume":"35","author":[{"given":"Tzu\u2010Yue","family":"Shiu","sequence":"first","affiliation":[{"name":"Division of Gastroenterology Department of Internal Medicine Tri\u2010Service General Hospital National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin\u2010Hung","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Department of Internal Medicine Tri\u2010Service General Hospital National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsuan\u2010Hwai","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Department of Internal Medicine Tri\u2010Service General Hospital National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Lueng","family":"Shih","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Department of Internal Medicine Tri\u2010Service General Hospital National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng\u2010Cheng","family":"Chu","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Department of Internal Medicine Tri\u2010Service General Hospital National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Kuo","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Department of Internal Medicine Tri\u2010Service General Hospital National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsai\u2010Yuan","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Department of Internal Medicine Tri\u2010Service General Hospital National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(00)80268-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199511023331802"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.12.2.152"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91273-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.40.1.581"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.22.12128"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1203\/pdr.0b013e31805365c5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108877"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21105"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2010.07.004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2217\/14622416.9.6.703"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/03602530903209429"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.07.3031"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.fpc.0000173483.13689.56"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000133513"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1004(200010)16:4<297::AID-HUMU2>3.0.CO;2-Z"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/FPC.0b013e328012d0da"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008571-199704000-00010"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008571-199808000-00010"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.14.8170"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.1999.01984.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(98)70408-1"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91266-0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/4.7.1183"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)90702-5"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)92514-7"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.103.6.1224"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20177"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.4238\/2014.January.28.12"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(87)90171-9"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008571-200008000-00007"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2001.03699.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1996.d01-1937.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2001.0349"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2002.6683"}],"container-title":["Liver International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fliv.12785","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fliv.12785","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/liv.12785","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T13:05:19Z","timestamp":1695560719000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/liv.12785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,6]]},"references-count":35,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/liv.12785"],"URL":"https:\/\/doi.org\/10.1111\/liv.12785","archive":["Portico"],"relation":{},"ISSN":["1478-3223","1478-3231"],"issn-type":[{"value":"1478-3223","type":"print"},{"value":"1478-3231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,6]]}}}