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Mineralocorticoid receptor (<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>) activation could play a role in hepatic fibrogenesis and its modulation could be beneficial for <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate whether eplerenone, a specific <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> antagonist, ameliorates liver damage in experimental <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C57bl6 mice were fed a choline\u2010deficient and amino acid\u2010defined (<jats:styled-content style=\"fixed-case\">CDAA<\/jats:styled-content>) diet for 22\u00a0weeks with or without eplerenone supplementation. Serum levels of aminotransferases and aldosterone were measured and hepatic steatosis, inflammation and fibrosis scored histologically. Hepatic triglyceride content (<jats:styled-content style=\"fixed-case\">HTC<\/jats:styled-content>) and hepatic <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of pro\u2010inflammatory pro\u2010fibrotic, oxidative stress\u2010associated genes and of <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> were also assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CDAA<\/jats:styled-content> diet effectively induced fibrotic <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>, and increased the hepatic expression of pro\u2010inflammatory, pro\u2010fibrotic and oxidative stress\u2010associated genes. Hepatic <jats:styled-content style=\"fixed-case\">MR mRNA<\/jats:styled-content> levels significantly correlated with the expression of pro\u2010inflammatory and pro\u2010fibrotic genes and were significantly increased in hepatic stellate cells obtained from <jats:styled-content style=\"fixed-case\">CDAA<\/jats:styled-content>\u2010fed animals. Eplerenone administration was associated to a reduction in histological steatosis and attenuation of liver fibrosis development, which was associated to a significant decrease in the expression of collagen\u2010\u03b11, collagen type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>, alpha 1 and <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>atrix metalloproteinase\u20102.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expression of <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> correlates with inflammation and fibrosis development in experimental <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>. Specific <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> blockade with eplerenone has hepatic anti\u2010steatotic and anti\u2010fibrotic effects. These data identify eplerenone as a potential novel therapy for <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>. Considering its safety and <jats:styled-content style=\"fixed-case\">FDA<\/jats:styled-content>\u2010approved status, human studies are warranted.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12794","type":"journal-article","created":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T13:44:51Z","timestamp":1422884691000},"page":"2129-2138","source":"Crossref","is-referenced-by-count":62,"title":["Beneficial effects of mineralocorticoid receptor blockade in experimental non\u2010alcoholic steatohepatitis"],"prefix":"10.1111","volume":"35","author":[{"given":"Margarita","family":"Pizarro","sequence":"first","affiliation":[{"name":"Departamento de Gastroenterolog\u00eda Escuela de Medicina Pontificia Universidad Cat\u00f3lica de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Sol\u00eds","sequence":"additional","affiliation":[{"name":"Departamento de Gastroenterolog\u00eda Escuela de Medicina Pontificia Universidad Cat\u00f3lica de 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