{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:33:38Z","timestamp":1762176818236},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,23]],"date-time":"2015-07-23T00:00:00Z","timestamp":1437609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001062","name":"Wallace H. Coulter Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008503","name":"Center for Integration of Medicine and Innovative Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>Early detection of portal hypertension (<jats:styled-content style=\"fixed-case\">PH<\/jats:styled-content>) may help to prevent the morbidity of late\u2010stage cirrhosis by stratifying disease severity and enabling disease\u2010modifying interventions in potentially reversible conditions like non\u2010alcoholic fatty liver disease and alcoholic hepatitis. This study seeks to correlate morphometric features by confocal endomicroscopy with established surrogate clinical markers of <jats:styled-content style=\"fixed-case\">PH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with and without <jats:styled-content style=\"fixed-case\">PH<\/jats:styled-content> scheduled for upper endoscopy at VA Boston participated in this <jats:styled-content style=\"fixed-case\">IRB<\/jats:styled-content>\u2010approved study. Real\u2010time probe\u2010based confocal endomicroscopy (<jats:styled-content style=\"fixed-case\">pCLE<\/jats:styled-content>) was performed in the duodenum. Vascular and epithelial morphometry was performed off\u2010line, in a blinded manner, using image\u2010processing software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Morphometric analysis of <jats:styled-content style=\"fixed-case\">pCLE<\/jats:styled-content> images from 16 patients with <jats:styled-content style=\"fixed-case\">PH<\/jats:styled-content> and 15 control patients was performed. Statistically significant differences were observed among control and <jats:styled-content style=\"fixed-case\">PH<\/jats:styled-content> patients for average vessel diameter (<jats:styled-content style=\"fixed-case\">AVD<\/jats:styled-content>: 11.7\u00a0\u03bcm vs. 17.1\u00a0\u03bcm), average vessel branching (<jats:styled-content style=\"fixed-case\">AVB<\/jats:styled-content>: 0.11 vs. 0.31 bifurcations per image frame), and average columnar cell height (<jats:styled-content style=\"fixed-case\">ACCH<\/jats:styled-content>: 40.0\u00a0\u03bcm vs. 52.0\u00a0\u03bcm). Spearman correlations comparing <jats:styled-content style=\"fixed-case\">AVD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> AVB<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ACCH<\/jats:styled-content> to portal gastropathy scores (0.86, 0.44 and 0.70) and to grade of oesophageal varices (0.88, 0.41 and 0.66) were statistically significant. Similarly, Pearson correlations of <jats:styled-content style=\"fixed-case\">AVD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ACCH<\/jats:styled-content> to spleen size (0.72 and 0.57), platelet count (\u22120.69 and \u22120.40) and the platelet count\/spleen size ratio (\u22120.69 and \u22120.41) were also found to be statistically significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Duodenal <jats:styled-content style=\"fixed-case\">pCLE<\/jats:styled-content> reveals microvascular dilatation and altered epithelial cell volume\/morphology in PH. These morphometric <jats:styled-content style=\"fixed-case\">pCLE<\/jats:styled-content> markers correlate with surrogate markers of <jats:styled-content style=\"fixed-case\">PH<\/jats:styled-content>. Additional studies will define the correlation between microscopic vascular patterns, epithelial cell volume and the hepatic venous pressure gradient.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.12906","type":"journal-article","created":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T07:31:00Z","timestamp":1435822260000},"page":"223-231","source":"Crossref","is-referenced-by-count":5,"title":["Probe\u2010based confocal laser endomicroscopy quantitative morphometric markers associated with portal hypertension in duodenal mucosa"],"prefix":"10.1111","volume":"36","author":[{"given":"Eladio","family":"Rodriguez\u2010Diaz","sequence":"first","affiliation":[{"name":"Department of Medicine Section\u00a0of Gastroenterology Boston University School of Medicine Boston MA USA"},{"name":"Section\u00a0of Gastroenterology VA Boston Healthcare System Boston MA 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