{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:53:44Z","timestamp":1790834024527,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,8,20]],"date-time":"2017-08-20T00:00:00Z","timestamp":1503187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["DZIF TTU\u201005\u2010702"],"award-info":[{"award-number":["DZIF TTU\u201005\u2010702"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG SU 888\/1\u20101"],"award-info":[{"award-number":["DFG SU 888\/1\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2018,2]]},"abstract":"Abstract<\/jats:title>\n \n Background and Aims<\/jats:title>\n \n Hepatitis E virus (\n HEV<\/jats:styled-content>\n ) is a major cause of acute viral hepatitis with >3 million symptomatic cases per year accounting for 70\u00a0000\n HEV<\/jats:styled-content>\n \u2010related deaths.\n HEV<\/jats:styled-content>\n \u2010specific T\u2010cell responses have been investigated against structural proteins expressed by open reading frames (\n ORF<\/jats:styled-content>\n ) 2 and 3. T\u2010cell responses against non\u2010structural\n HEV<\/jats:styled-content>\n proteins encoded by\n ORF<\/jats:styled-content>\n 1 are hardly studied. The aim of this study was to determine\n HEV ORF<\/jats:styled-content>\n 1\u2010specific T\u2010cell responses in comparison to\n ORF<\/jats:styled-content>\n 2\/3 in patients exposed to\n HEV<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n HEV<\/jats:styled-content>\n \u2010specific\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n and\n CD<\/jats:styled-content>\n 8\n +<\/jats:sup>\n T\u2010cell responses against\n HEV<\/jats:styled-content>\n genotype 3 were investigated in patients with acute and chronic hepatitis E as well as in\n HEV<\/jats:styled-content>\n seropositive and seronegative individuals.\n HEV<\/jats:styled-content>\n \u2010specific T\u2010cell responses were determined by proliferation and intracellular cytokine assay upon stimulation of\n PBMC<\/jats:styled-content>\n s with\n HEV<\/jats:styled-content>\n \u2010specific overlapping peptide pools spanning the entire\n HEV<\/jats:styled-content>\n genome.\n HEV<\/jats:styled-content>\n \u2010antigen was measured using an anti\u2010\n HEV<\/jats:styled-content>\n antigen\u2010specific\n ELISA<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Broad\n HEV ORF<\/jats:styled-content>\n 1\u2010specific T\u2010cell responses were detected in patients with acute, resolved and chronic hepatitis E without distinct dominant regions. The magnitude and frequency in recognition of\n ORF<\/jats:styled-content>\n 1\u2010specific T\u2010cell responses were similar compared to responses against\n HEV ORF<\/jats:styled-content>\n 2\/3. Longitudinal studies of\n HEV<\/jats:styled-content>\n \u2010specific T\u2010cell responses displayed similar behaviour against structural and non\u2010structural proteins.\n HEV<\/jats:styled-content>\n \u2010antigen levels were inversely correlated with\n HEV<\/jats:styled-content>\n \u2010specific T\u2010cell responses.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n HEV<\/jats:styled-content>\n \u2010specific T\u2010cell responses are detectable against the entire\n HEV<\/jats:styled-content>\n genome including the non\u2010structural proteins.\n HEV<\/jats:styled-content>\n \u2010specific T\u2010cell responses are associated with control of\n HEV<\/jats:styled-content>\n infection. These findings have implications for the design of\n HEV<\/jats:styled-content>\n vaccines.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/liv.13521","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T11:32:39Z","timestamp":1500377559000},"page":"266-277","source":"Crossref","is-referenced-by-count":24,"title":["Hepatitis E virus\n ORF<\/scp>\n 1 induces proliferative and functional T\u2010cell responses in patients with ongoing and resolved hepatitis E"],"prefix":"10.1111","volume":"38","author":[{"given":"Jana","family":"Al\u2010Ayoubi","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Behrendt","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Bremer","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pothakamuri Venkata","family":"Suneetha","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anett","family":"Gisa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Rinker","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Manns","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"},{"name":"German Center for Infection Research (DZIF) Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Cornberg","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"},{"name":"German Center for Infection Research (DZIF) Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2906-0480","authenticated-orcid":false,"given":"Heiner","family":"Wedemeyer","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical School Hannover Germany"},{"name":"German Center for Infection Research (DZIF) Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9666-0753","authenticated-orcid":false,"given":"Anke R. 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