{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T20:27:26Z","timestamp":1789244846614,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T00:00:00Z","timestamp":1505779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Beijing Municipal Funds for the Research Work of Featured Clinically Applicable Projects","award":["(Z141107006614008)"],"award-info":[{"award-number":["(Z141107006614008)"]}]},{"name":"Tianjin Municipal Science and Technology Special Funds for Enterprise","award":["(14ZXLJSY00320)"],"award-info":[{"award-number":["(14ZXLJSY00320)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content> is a prototypical autoimmune liver disease characterized by portal lymphoplasmacyte infiltration. <jats:styled-content style=\"fixed-case\">ALD<\/jats:styled-content> is a prototypical environment\u2010driven disease, featured by mild lymphocyte infiltration. We hypothesize that B cells are more involved in the pathogenesis of <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>. By analysing the infiltrating B cell repertoire, we aimed to unveil greater oligoclonal expansion and active clonal exchange between liver and periphery in <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content> than in <jats:styled-content style=\"fixed-case\">ALD<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content> of Ig H chain genes, we analysed the liver\u2010infiltrating and paired peripheral B lymphocyte repertoire from nine <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content> and four <jats:styled-content style=\"fixed-case\">ALD<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the liver of <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ALD<\/jats:styled-content> patients, (i) roughly 10% of the B lymphocytes were clonally related and highly expressed, and there were also lineages that underwent extensive clonal expansion; (ii) there was different use of <jats:styled-content style=\"fixed-case\">IGHV<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">IGHJ<\/jats:styled-content> segments between <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ALD<\/jats:styled-content>, suggesting distinct Ag exposure backgrounds, but this did not lead to a significant difference in their clonal expansion level. Analysis of data sets from paired samples further revealed, (iii) direct clonal exchange and evolutionally related B cell clones between the infiltrating and peripheral repertoire; (iv) the seeding of the infiltrating clones to periphery, and peripheral ones to the liver, for further extensive evolution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The oligoclonally expanded nature of the infiltrating B cell repertoire implies B cell immunity is involved in the pathogenesis of both diseases. The observed clonal exchange might provide an approach to identify and monitor the infiltrating B cells through the periphery.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.13554","type":"journal-article","created":{"date-parts":[[2017,8,19]],"date-time":"2017-08-19T09:52:38Z","timestamp":1503136358000},"page":"542-552","source":"Crossref","is-referenced-by-count":7,"title":["Clonal characteristics of paired infiltrating and circulating B lymphocyte repertoire in patients with primary biliary cholangitis"],"prefix":"10.1111","volume":"38","author":[{"given":"Yan\u2010guo","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory FuXing Hospital Capital Medical University  Beijing China"},{"name":"Beijing You An Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Translational Genomics Center BGI\u2010Tianjin  Tianjin China"},{"name":"BGI\u2010Shenzhen  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Translational Genomics Center BGI\u2010Tianjin  Tianjin China"},{"name":"Tianjin Marvelbio Technology Co. 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