{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:31:17Z","timestamp":1765546277417,"version":"3.37.3"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T00:00:00Z","timestamp":1516838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s are important genetic regulators of physiological and pathophysiological processes including cancer initiation and progression of hepatoblastoma, the most common liver tumour in childhood. We aimed to identify malignant and metastasis promoting effects of miR\u2010492, a mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, previously reported to be overexpressed in metastatic hepatoblastoma. Furthermore, we intended to evaluate its diagnostic and prognostic potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Stable and transient overexpression of miR\u2010492 in two liver tumour cell lines HepT1 and <jats:styled-content style=\"fixed-case\">HUH<\/jats:styled-content>7 was used to analyse features of metastatic tumour progression such as proliferation, anchorage\u2010independent growth, migration and invasion. Via a mass spectrometry based proteomic screen, we investigated mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010492\u2010dependent effects on proteome level and explored the underlying biology. One of the predicted target genes, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>44, was experimentally validated via luciferase assays. Diagnostic and prognostic properties of miR\u2010492 were studied in hepatoblastoma tumour samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that miR\u2010492 significantly enhances cell proliferation, anchorage\u2010independent growth, migration and invasion of hepatoblastoma cells. We also identified and validated <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>44, a transmembrane adhesion receptor for hyaluronan, as direct and functional target of miR\u2010492. This mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> has a strong direct impact on two <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>44 isoforms (standard and v10). High miR\u2010492 expression correlates with high\u2010risk or aggressive tumours and further bears potential for predicting reduced event\u2010free survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We identified miR\u2010492 and its target <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>44 as regulators of a number of biological features important for malignancy and metastasis. Furthermore, we demonstrated the diagnostic and prognostic potential of miR\u2010492, a promising novel therapeutic target and biomarker for hepatoblastoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.13687","type":"journal-article","created":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T13:07:50Z","timestamp":1514984870000},"page":"1280-1291","source":"Crossref","is-referenced-by-count":32,"title":["MiR\u2010492 regulates metastatic properties of hepatoblastoma via <scp>CD<\/scp>44"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5522-2805","authenticated-orcid":false,"given":"Julia","family":"von Frowein","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Children's Research Center Dr. von Hauner Children's Hospital Ludwig\u2010Maximilians\u2010University Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie 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